Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry
文献类型：期刊论文
| 作者 | Zhang, Junjie1; Zhao, Chunxia1; Chang, Yuwei1; Zhao, Yanni1; Li, Qinghua1,2; Lu, Xin1; Xu, Guowang1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 36期号:17页码:2868-2877 |
| 关键词 | Free amino acids Single quadrupole mass spectrometry Tobacco Ultra-high performance liquid chromatography |
| 产权排序 | 待补充 |
| 通讯作者 | 许国旺 |
| 合作状况 | 英 |
| 英文摘要 | amino acids are one of the most important metabolites of organisms. they play an important role in plant growth, development, and product quality. a method based on rp ultra-performance lc with single quadrupole ms and 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate precolumn derivatization was developed for the analysis of free amino acids in flue-cured tobacco leaves. unlike the corresponding uv detection method, this method avoids matrix interference of complicated tobacco components, and the quantitative accuracy and resolution were improved. twenty free amino acids were detected in flue-cured tobacco leaves. the method showed a good linearity with correlation coefficients of 0.9966-0.9998. the lods for derivatized amino acids were 0.2-9.7 fmol/l. good repeatability with an rsd of 2.5-8.6% and satisfactory intra- and interday precisions were obtained. the developed method was used to investigate free amino acids in flue-cured tobacco leaves in china. the effects of aroma type, variety, and growing regions on free amino acids were investigated. the results showed that free amino acids in tobacco were affected by growing regions and varieties. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | precolumn derivatization ; ms/ms analysis ; ms ; metabolism ; diagnosis ; hplc ; tool |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 2877 |
| 语种 | 英语 |
| WOS记录号 | WOS:000323891300018 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119517]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Hongyun Honghe Tobacco Grp, Technol Ctr, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Junjie,Zhao, Chunxia,Chang, Yuwei,et al. Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry[J]. journal of separation science,2013,36(17):2868-2877. |
| APA | Zhang, Junjie.,Zhao, Chunxia.,Chang, Yuwei.,Zhao, Yanni.,Li, Qinghua.,...&Xu, Guowang.(2013).Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry.journal of separation science,36(17),2868-2877. |
| MLA | Zhang, Junjie,et al."Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry".journal of separation science 36.17(2013):2868-2877. |
入库方式： OAI收割
来源：大连化学物理研究所
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