A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis
文献类型：期刊论文
| 作者 | Wang, Shuangyuan; Li, Jia; Shi, Xianzhe; Qiao, Lizhen; Lu, Xin; Xu, Guowang |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2013-12-20 |
| 卷号 | 1321页码:65-72 |
| 关键词 | Stop-flow On-line Two-dimensional liquid chromatography Lipid MS |
| 产权排序 | 待补充 |
| 通讯作者 | 石先哲 ; 许国旺 |
| 合作状况 | 英 |
| 英文摘要 | a novel on-line two dimensional liquid chromatography (2d lc) based on stop-flow mode coupled with electrospray ionization mass spectrometry (esi-ms) method was established to separate lipids in human plasma. hydrophilic interaction liquid chromatography (hilic) in the first dimension and reversed-phase liquid chromatography (rp lc) in the second dimension were used to separate the lipids into six fractions based on their polar head groups and further into peaks based on aliphatic chains, respectively. a new stop-flow interface with a trap column and an extra make-up flow was designed to construct this system and trap the components eluted from the first dimension. moreover, the same length of analytical columns and similar flow rates were used in the first and second dimensions. therefore, the new stop-flow 2d lc system can avoid the sensitivity decrease caused by the dilution effect, which is the shortcoming of comprehensive 2d lc. three hundred and seventy-two lipids were identified from plasma extract using this 2d lc coupled with esi-ms in positive mode, and 88 more lipids were detected than one-dimensional rp lc analysis. peak capacity of this stop-flow 2d lc was 415, which is similar to that of comprehensive 2d lc. the linearity, repeatability and sensitivity of this method were satisfactory, which demonstrated that this method was also suitable for quantitative analysis. all these results indicated that this on-line 2d lc method is powerful for qualitative and quantitative analysis of complex lipids (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | hydrophilic interaction ; phospholipid classes ; biological samples ; crude extracts ; human blood ; separation ; plasma ; identification ; temperature ; rplc |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 72 |
| 语种 | 英语 |
| WOS记录号 | WOS:000328596400008 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119533]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shuangyuan,Li, Jia,Shi, Xianzhe,et al. A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis[J]. journal of chromatography a,2013,1321:65-72. |
| APA | Wang, Shuangyuan,Li, Jia,Shi, Xianzhe,Qiao, Lizhen,Lu, Xin,&Xu, Guowang.(2013).A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis.journal of chromatography a,1321,65-72. |
| MLA | Wang, Shuangyuan,et al."A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis".journal of chromatography a 1321(2013):65-72. |
入库方式： OAI收割
来源：大连化学物理研究所
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