Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow
文献类型：期刊论文
| 作者 | Bian, Yangyang1,2; Ye, Mingliang1; Wang, Chunli1,2; Cheng, Kai1,2; Song, Chunxia1,2; Dong, Mingming1,2; Pan, Yanbo1,2; Qin, Hongqiang1,2; Zou, Hanfa1 |
| 刊名 | scientific reports
 |
| 出版日期 | 2013-12-10 |
| 卷号 | 3页码:03460 |
| ISSN号 | 2045-2322 |
| 产权排序 | 待补充 |
| 通讯作者 | 叶明亮 ; 邹汉法 |
| 合作状况 | 英 |
| 英文摘要 | due to its constitutive activity and ubiquitous distribution, ck2 is the most pleiotropic kinase among the individual members of the protein kinase superfamily. identification of ck2 substrates is vital to decipher its role in biological processes. however, only a limited number of ck2 substrates were identified so far. in this study, we developed an integrated phosphoproteomics workflow to identify the ck2 substrates in large scale. first, in vitro kinase reactions with immobilized proteomes were combined with quantitative phosphoproteomics to identify in vitro ck2 phosphorylation sites, which leaded to identification of 988 sites from 581 protein substrates. to reduce false positives, we proposed an approach by comparing these in vitro sites with the public databases that collect in vivo phosphorylation sites. after the removal of the sites that were excluded in the databases, 605 high confident ck2 sites corresponding to 356 proteins were retained. the ck2 substrates identified in this study were based on the discovery mode, in which an unbiased overview of ck2 substrates was provided. our result revealed that ck2 substrates were significantly enriched in the spliceosomal proteins, indicating ck2 might regulate the functions of spliceosome. |
| WOS标题词 | science & technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | protein-phosphorylation ; comprehensive resource ; identification ; spliceosome ; complexes ; reveals ; atlas ; mouse ; assay |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 03460 |
| 语种 | 英语 |
| WOS记录号 | WOS:000328125600003 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119581]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bian, Yangyang,Ye, Mingliang,Wang, Chunli,et al. Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow[J]. scientific reports,2013,3:03460. |
| APA | Bian, Yangyang.,Ye, Mingliang.,Wang, Chunli.,Cheng, Kai.,Song, Chunxia.,...&Zou, Hanfa.(2013).Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow.scientific reports,3,03460. |
| MLA | Bian, Yangyang,et al."Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow".scientific reports 3(2013):03460. |
入库方式： OAI收割
来源：大连化学物理研究所
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