An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus
文献类型：期刊论文
| 作者 | Zhong, Weiliang1; Ma, Huipeng2; Wang, Shouyu1; Gao, Xinghua2; Zhang, Weiguo1; Qin, Jianhua2 |
| 刊名 | microfluidics and nanofluidics
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 15期号:6页码:763-773 |
| 关键词 | Microfluidic Flow shear stress Chondrocyte Mechanical microenvironment |
| ISSN号 | 1613-4982 |
| 产权排序 | 待补充 |
| 通讯作者 | weiguo zhang |
| 合作状况 | 英 |
| 英文摘要 | in this work, we presented a novel integrated microfluidic perfusion system to generate multiple parameter fluid flow-induced shear stresses simultaneously and investigated the effects of distinct levels of fluid flow stimulus on the responses of chondrocytes, including the changes of morphology and metabolism. based on the electric circuit analogy, two devices were fabricated, each with four chambers to enable eight different shear stresses spanning over four orders of magnitude from 0.007 to 15.4 dyne/cm(2) with computational fluid dynamics analysis. chondrocytes subjected to shear stresses (7.5 and 15.4 dyne/cm(2)) for 24 h reoriented their cytoskeleton to align with the direction of flow. meanwhile, the collagen i, collagen ii and aggrecan expression of chondrocytes increased in different ranges, respectively. furthermore, interleukin-6 as a proinflammatory cytokine can be detected at shear stress of 7.5 and 15.4 dyne/cm(2) in mrna level. these results indicated that fluid flow was beneficial for chondrocyte metabolism at interstitial levels (0.007 and 0.046 dyne/cm(2)), but induced an increase in fibrocartilage phenotype with increasing magnitude of stimulation. moreover, a moderate level of flow stimulus (7.5 dyne/cm(2)) could also result in detrimental cytokine release. this work described a simple and versatile way to rapidly screen cell responses to fluid flow stimulus from interstitial shear stress level to pathological level, providing multi-condition fluid flow-induced microenvironment in vitro for understanding deeply chondrocyte metabolism, cartilage reconstruction and osteoarthritis etiology. |
| WOS标题词 | science & technology ; technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | nanoscience & nanotechnology ; instruments & instrumentation ; physics, fluids & plasmas |
| 研究领域[WOS] | science & technology - other topics ; instruments & instrumentation ; physics |
| 关键词[WOS] | human osteoarthritic chondrocytes ; tensile mechanical-properties ; shear-stress ; articular-cartilage ; interstitial flow ; nitric-oxide ; in-vitro ; tissue ; cells ; expression |
| 收录类别 | SCI |
| 资助信息 | 2，2 |
| 原文出处 | 773 |
| 语种 | 英语 |
| WOS记录号 | WOS:000327131200004 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119635]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Dept Orthopaed, Affiliated Hosp 1, Dalian, Peoples R China 2.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Weiliang,Ma, Huipeng,Wang, Shouyu,et al. An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus[J]. microfluidics and nanofluidics,2013,15(6):763-773. |
| APA | Zhong, Weiliang,Ma, Huipeng,Wang, Shouyu,Gao, Xinghua,Zhang, Weiguo,&Qin, Jianhua.(2013).An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus.microfluidics and nanofluidics,15(6),763-773. |
| MLA | Zhong, Weiliang,et al."An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus".microfluidics and nanofluidics 15.6(2013):763-773. |
入库方式： OAI收割
来源：大连化学物理研究所
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