An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots
文献类型：期刊论文
| 作者 | Sui, Tao1; Ge, Yingbin2; Liu, Wujun3; Zhao, Zongbao K.3; Zhang, Ning1; Cao, Xiaojian1 |
| 刊名 | plos one
 |
| 出版日期 | 2013-07-23 |
| 卷号 | 8期号:7页码:69049 |
| 产权排序 | 待补充 |
| 通讯作者 | 赵宗保 ; zhang n ; cao xj |
| 合作状况 | 英 |
| 英文摘要 | differences in the levels of acetylcholinesterase (ache) in ventral and dorsal spinal roots can be used to differentiate the spinal nerves. although many methods are available to assay ache, a rapid and sensitive method has not been previously developed. here, we describe an antibody-based quartz crystal microbalance (qcm) assay and its application for the quantification of ache in the solutions of ventral and dorsal spinal roots. the frequency variation of the qcm device corresponds to the level of ache over a wide dynamic range (0.5-10 mu g/ml), which is comparable to the response range of the elisa method. the frequency shift caused by the ventral roots is 3-fold greater than that caused by the dorsal roots. the antibody-based qcm sensor was stable across many successive replicate samples, and the method required less than 10 min, including the ache extraction and analysis steps. this method is a rapid and convenient means for the quantification of ache in biological samples and may be applicable for distinguishing the ventral and dorsal roots during surgical operations. |
| WOS标题词 | science & technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | peripheral-nerve ; histochemical identification ; immobilization methods ; sensory fascicles ; motor ; dissipation ; neurons ; immunohistochemistry ; nanoparticles ; immunosensor |
| 收录类别 | SCI |
| 资助信息 | 3，3 |
| 原文出处 | 69049 |
| 语种 | 英语 |
| WOS记录号 | WOS:000325211000071 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119668]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nanjing Med Univ, Affiliated Hosp 1, Dept Orthoped, Nanjing, Jiangsu, Peoples R China 2.Nanjing Med Univ, Dept Physiol, Nanjing, Jiangsu, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sui, Tao,Ge, Yingbin,Liu, Wujun,et al. An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots[J]. plos one,2013,8(7):69049. |
| APA | Sui, Tao,Ge, Yingbin,Liu, Wujun,Zhao, Zongbao K.,Zhang, Ning,&Cao, Xiaojian.(2013).An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots.plos one,8(7),69049. |
| MLA | Sui, Tao,et al."An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots".plos one 8.7(2013):69049. |
入库方式： OAI收割
来源：大连化学物理研究所
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