Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood
文献类型：期刊论文
| 作者 | Cao, Jiali1; Zhang, Xihao1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2 |
| 刊名 | journal of materials chemistry b
 |
| 出版日期 | 2013 |
| 卷号 | 1期号:30页码:3625-3632 |
| 产权排序 | 待补充 |
| 通讯作者 | 陈朗星 ; 张玉奎 |
| 合作状况 | 英 |
| 英文摘要 | in this study, a facile and efficient separation of abundant proteins from bovine blood using core-shell structure nanoparticles with a magnetic core and an immobilized metal affinity ligand iminodiacetic acid (ida) chelating ni(ii) is presented. firstly, fe3o4 magnetic nanoparticles (mnps) were synthesized through a solvothermal method and then were conveniently surface-modified with 3-(methacryloyloxy) propyltrimethoxylsilane as anchor molecules to donate vinyl groups. next a high density poly(4-vinylbenzylchloride) (pvbc) shell was synthesized on the surface of silica-coated fe3o4 mnps via distillation-precipitation polymerization. after the pvbc shell reacted with iminodiacetic acid (ida) in alkaline aqueous solution, the magnetite was charged with ni2+ to form ni(ii)-ida functionalized hybrid fe3o4@pvbc@ida-ni mnps. transmission electron microscopy (tem), x-ray photoelectron spectroscopy (xps), x-ray diffraction (xrd), fourier transform infrared spectroscopy (ft-ir), thermogravimetric analysis (tga) and a vibrating sample magnetometer (vsm) were employed to evaluate the size, morphology and magnetic property of the resulting magnetic nanospheres. the high saturation magnetization (48.1 emu g(-1)) provides the materials with the convenience of magnetic separation under an external magnetic field and they can be subsequently reused. the core-shell fe3o4@pvbc@ida-ni mnps exhibit excellent performance in the separation of protein bovine hemoglobin (bhb), and the binding capacity is as high as 1988 mg g(-1). in addition, the fe3o4@pvbc@ida-ni mnps can be used in selective removal of abundant protein hb in the bovine blood samples. this opens a novel route for its future application in removing abundant protein in proteomic analysis. |
| WOS标题词 | science & technology ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | materials science, biomaterials |
| 研究领域[WOS] | materials science |
| 关键词[WOS] | histidine-tagged proteins ; core/shell nanoparticles ; biomedical applications ; microspheres ; chromatography ; extraction ; separation ; fe3o4/au ; surface ; samples |
| 收录类别 | SCI |
| 资助信息 | 2，5 |
| 原文出处 | 3632 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321626200001 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119674]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Jiali,Zhang, Xihao,He, Xiwen,et al. Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood[J]. journal of materials chemistry b,2013,1(30):3625-3632. |
| APA | Cao, Jiali,Zhang, Xihao,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2013).Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood.journal of materials chemistry b,1(30),3625-3632. |
| MLA | Cao, Jiali,et al."Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood".journal of materials chemistry b 1.30(2013):3625-3632. |
入库方式： OAI收割
来源：大连化学物理研究所
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