Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions
文献类型：期刊论文
| 作者 | Yang, F.1; Mao, J.1; He, X. W.1; Chen, L. X.1; Zhang, Y. K.1,2 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2013-08-01 |
| 卷号 | 405期号:21页码:6639-6648 |
| 关键词 | Boronate affinity chromatography Hybrid monolith Glycoprotein Physiological pH Separation |
| 产权排序 | 待补充 |
| 通讯作者 | 陈朗星 ; 张玉奎 |
| 合作状况 | 英 |
| 英文摘要 | in this study, a boronate-silica hybrid affinity monolith was prepared for specific capture of glycoproteins at neutral ph condition. the monolith was synthesized via a facile one-pot procedure in a stainless steel column by concurrently mixing hydrolyzed alkoxysilanes tetramethoxysilane and vinyltrimethoxysilane, organic monomer 3-acrylamidophenylboronic acid and initiator 2,2'-azobisisobutyronitrile together. the polycondensation of alkoxysilanes and copolymerization of organic monomer and vinyl-silica monolith were carried out successively by reacting at different temperatures. after optimizing the preparation conditions, the resulting hybrid affinity monolith was systematically characterized and exhibited excellent affinity to both cis-diol-containing small molecules and glycoproteins at neutral and physiological ph, including adenosine, horseradish peroxidase, transferrin and ovalbumin. the binding capacity of ovalbumin on monolith was measured to be 2.5 mg g(-1) at ph 7.0. furthermore, the hybrid affinity monolith was applied to the separation of transferrin from bovine serum sample at a physiological condition. good repeatability was obtained and the relative standard deviations of retention time were 1.15 and 4.77 % (n = 5) for run-to-run and column-to-column, respectively. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | capillary liquid-chromatography ; maldi-tof ms ; cis-diol ; mass-spectrometry ; column ; glycopeptides ; enrichment ; glycosylation ; separation ; electrochromatography |
| 收录类别 | SCI |
| 资助信息 | 2，5 |
| 原文出处 | 6648 |
| 语种 | 英语 |
| WOS记录号 | WOS:000322705600004 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119677]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, F.,Mao, J.,He, X. W.,et al. Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions[J]. analytical and bioanalytical chemistry,2013,405(21):6639-6648. |
| APA | Yang, F.,Mao, J.,He, X. W.,Chen, L. X.,&Zhang, Y. K..(2013).Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions.analytical and bioanalytical chemistry,405(21),6639-6648. |
| MLA | Yang, F.,et al."Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions".analytical and bioanalytical chemistry 405.21(2013):6639-6648. |
入库方式： OAI收割
来源：大连化学物理研究所
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