An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome
文献类型：期刊论文
| 作者 | Bian, Yangyang1,2; Song, Chunxia1,2; Cheng, Kai1,2; Dong, Mingming1,2; Wang, Fangjun1; Huang, Junfeng1,2; Sun, Deguang3; Wang, Liming3; Ye, Mingliang1; Zou, Hanfa1 |
| 刊名 | journal of proteomics
 |
| 出版日期 | 2014-01-16 |
| 卷号 | 96页码:253-262 |
| 关键词 | Human liver phosphoproteome RP-RPLC Glu-C digestion TripleTOF 5600 LTQ Orbitrap Velos Mass spectrometer |
| ISSN号 | 1874-3919 |
| 产权排序 | 待补充 |
| 通讯作者 | 叶明亮 ; 邹汉法 |
| 合作状况 | 英 |
| 英文摘要 | protein phosphorylation is one of the most common post-translational modifications. it plays key roles in regulating diverse biological processes of liver tissues. to better understand the role of protein phosphorylation in liver functions, it is essential to perform in-depth phosphoproteome analysis of human liver. here, an enzyme assisted reversed-phase-reversed-phase liquid chromatography (rp-rplc) approach with both rplc separations operated with optimized acidic mobile phase was developed. high orthogonal separation was achieved by trypsin digestion of the glu-c generated peptides in the fractions collected from the first rplc separation. the phosphoproteome coverage was further improved by using two types of instruments, i.e. tripletof 5600 and ltq orbitrap velos. a total of 22,446 phosphorylation sites, corresponding to 6526 nonredundant phosphoproteins were finally identified from normal human liver tissues. of these sites, 15,229 sites were confidently localized with ascore >= 13. this dataset was the largest phosphoproteome dataset of human liver. it can be a public resource for the liver research community and holds promise for further biology studies. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | ion affinity-chromatography ; protein identification technology ; phosphorylated peptides ; mass-spectrometry ; multidimensional separation ; enrichment ; proteomics ; phosphopeptides ; strategy ; networks |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 262 |
| 语种 | 英语 |
| WOS记录号 | WOS:000331916300020 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119681]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bian, Yangyang,Song, Chunxia,Cheng, Kai,et al. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome[J]. journal of proteomics,2014,96:253-262. |
| APA | Bian, Yangyang.,Song, Chunxia.,Cheng, Kai.,Dong, Mingming.,Wang, Fangjun.,...&Zou, Hanfa.(2014).An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.journal of proteomics,96,253-262. |
| MLA | Bian, Yangyang,et al."An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome".journal of proteomics 96(2014):253-262. |
入库方式： OAI收割
来源：大连化学物理研究所
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