A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model
文献类型：期刊论文
| 作者 | Zhang, Min1; Li, Hongjing2; Ma, Huipeng3; Qin, Jianhua1 |
| 刊名 | wound repair and regeneration
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 21期号:6页码:897-903 |
| ISSN号 | 1067-1927 |
| 产权排序 | 待补充 |
| 通讯作者 | 秦建华 |
| 合作状况 | 英 |
| 英文摘要 | in vitro scratch wound assays are commonly used strategies to measure cell repair rate, facilitating the study of cell migration, tissue reorganization, and cell division. this work presented a simple and novel microfluidic device that allowed a quantitative investigation of the cell migration and cell proliferation behaviors in an in vitro wound-healing model, especially focused on the scratch assay. the microfluidic device is composed of four units, which include cell growth regions and cell-free regions created by micropillars. using this device, we evaluated the proliferation and migration process of human gastric epithelial cells in the presence of different concentrations of the epidermal growth factor, and investigated the migration behavior of mesenchymal stem cells toward tumor cells as well. this approach has the unique capability to create localized cell-free regions in parallel, and facilitate quantitative research on cell migration in the wound-healing process, providing a powerful platform for elucidating the mechanism of cell migration in regeneration medicine. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | cell biology ; dermatology ; medicine, research & experimental ; surgery |
| 研究领域[WOS] | cell biology ; dermatology ; research & experimental medicine ; surgery |
| 关键词[WOS] | motility ; complex |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 903 |
| 语种 | 英语 |
| WOS记录号 | WOS:000326327100162 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119682]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 1, Dept Orthoped, Dalian, Liaoning, Peoples R China 3.Dalian Med Univ, Coll Med Lab, Dalian, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Li, Hongjing,Ma, Huipeng,et al. A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model[J]. wound repair and regeneration,2013,21(6):897-903. |
| APA | Zhang, Min,Li, Hongjing,Ma, Huipeng,&Qin, Jianhua.(2013).A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model.wound repair and regeneration,21(6),897-903. |
| MLA | Zhang, Min,et al."A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model".wound repair and regeneration 21.6(2013):897-903. |
入库方式： OAI收割
来源：大连化学物理研究所
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