One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides
文献类型：期刊论文
| 作者 | Fang, Chunli1; Xiong, Zhichao2; Qin, Hongqiang3; Huang, Guang3; Liu, Jing3; Ye, Mingliang3; Feng, Shun1; Zou, Hanfa3 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2014-09-02 |
| 卷号 | 841页码:99-105 |
| 关键词 | Glycopeptides Magnetic colloidal nanocrystal clusters Chitosan Hydrophilic interaction liquid chromatography |
| ISSN号 | 0003-2670 |
| 产权排序 | 待补充 |
| 通讯作者 | 封顺 ; 邹汉法 |
| 合作状况 | 英 |
| 英文摘要 | selective enrichment of glycopeptides prior to the mass spectrometry (ms) analysis is essential due to ion suppression effect during ionization caused by the co-presence of non-glycosylated peptides. among the enrichment approaches, hydrophilic interaction liquid chromatography (hilic) based on magnetic separation has become a popular method in recent years. as the conventional synthesis procedures of these materials are tedious and time-consuming with at least four steps. herein, magnetic colloidal nanocrystal clusters coated with chitosan (fe3o4@cs mcncs) have been successfully prepared by a simple one-pot method. the resulting fe3o4@cs mcncs demonstrated an excellent ability for glycopeptide enrichment with high selectivity, low detection limit and high binding capacity. furthermore, in the analysis of real complicated biological sample, 283 unique n-glycosylation sites corresponding to 175 glycosylated proteins were identified in three replicate analyses of 45 mu g protein sample extracted from hela cells, indicating the great potential in detection and identification of low abundant glycopeptides in glycoproteome analysis. (c) 2014 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | interaction liquid-chromatography ; mass-spectrometry ; glycosylation ; nanoparticles ; polysaccharide ; identification ; silica |
| 收录类别 | SCI |
| 资助信息 | 3,3 |
| 原文出处 | 105 |
| 语种 | 英语 |
| WOS记录号 | WOS:000339990900013 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119694]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Xinjiang Univ, Coll Chem & Chem Engn, Minist Educ Xinjiang Uyghur Autonomous Reg, Key Lab Oil Gas Fine Chem, Urumqi 830046, Peoples R China 2.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Chunli,Xiong, Zhichao,Qin, Hongqiang,et al. One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides[J]. analytica chimica acta,2014,841:99-105. |
| APA | Fang, Chunli.,Xiong, Zhichao.,Qin, Hongqiang.,Huang, Guang.,Liu, Jing.,...&Zou, Hanfa.(2014).One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides.analytica chimica acta,841,99-105. |
| MLA | Fang, Chunli,et al."One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides".analytica chimica acta 841(2014):99-105. |
入库方式： OAI收割
来源：大连化学物理研究所
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