Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification
文献类型：期刊论文
| 作者 | Yang, Xiaomin2; Hu, Lianghai1,3; Ye, Mingliang3; Zou, Hanfa3 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2014-06-04 |
| 卷号 | 829页码:40-47 |
| 关键词 | Proteomics Peptidomics Human urine Nanoparticle Two-dimensional Biomarker |
| ISSN号 | 0003-2670 |
| 产权排序 | 待补充 |
| 通讯作者 | 胡良海 |
| 合作状况 | 英 |
| 英文摘要 | peptides in urine are excreted by kidney from the blood and tissues, which are composed of a large amount of hormones, cytokines, regulatory factors and the metabolized fragments of proteins. the peptide distribution in urine will reflect the physiological and pathophysiological processes in body. in past, limited information was reported about the composition of the peptides in urine. one possible reason is that the peptides in urine are fairly low abundant and there are high concentrations of salts and organic metabolites in the urine. in this report, we extracted the peptides from human urine by highly ordered mesoporous silica particles with the pore size of 2 nm, which will exclude the high molecular weight proteins over 12 kda. the extracted peptides were then separated into fractions according to their molecular weight by size exclusion chromatography. each of the fractions was further analyzed by maldi-tof ms and mu rplc-ms/ms. totally, 193 peptides were identified by two-dimensional sec/mu rplc-ms/ms analysis. by analyzing the progenitor protein of the peptides; we found that two-thirds of the proteins differed from the reported urine proteome database, and the high abundant proteins in urine proteome were less detected in the urine peptidome. the developed extraction and separation methods were efficient for the profiling of the endogenous peptides in human urine. the peptidome in human urine was complementary to the human urinary proteome and may provide an emerging field for biomarker discovery. published by elsevier b.v. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | human plasma ; biomarker discovery ; serum peptidome ; comprehensive analysis ; liquid-chromatography ; selective extraction ; protein-fragments ; mesoporous silica ; lc-ms/ms ; proteomics |
| 收录类别 | SCI |
| 资助信息 | 3，2 |
| 原文出处 | 47 |
| 语种 | 英语 |
| WOS记录号 | WOS:000336388700007 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119704]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Jilin, Peoples R China 2.Jilin Univ, Coll Chem, Changchun 130012, Jilin, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Xiaomin,Hu, Lianghai,Ye, Mingliang,et al. Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification[J]. analytica chimica acta,2014,829:40-47. |
| APA | Yang, Xiaomin,Hu, Lianghai,Ye, Mingliang,&Zou, Hanfa.(2014).Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification.analytica chimica acta,829,40-47. |
| MLA | Yang, Xiaomin,et al."Analysis of the human urine endogenous peptides by nanoparticle extraction and mass spectrometry identification".analytica chimica acta 829(2014):40-47. |
入库方式： OAI收割
来源：大连化学物理研究所
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