Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column
文献类型：期刊论文
| 作者 | Wei, Jie1; Shen, Aijin2; Wan, Huihui3; Yan, Jingyu2; Yang, Bingcheng1; Guo, Zhimou2; Zhang, Feifang1; Liang, Xinmiao1,2 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 37期号:14页码:1781-1787 |
| 关键词 | Aminoglycosides Hydrophilic interaction chromatography Separation selectivity Zwitterionic stationary phases |
| ISSN号 | 1615-9306 |
| 产权排序 | 待补充 |
| 通讯作者 | 郭志谋 |
| 合作状况 | 英 |
| 英文摘要 | hydrophilic interaction liquid chromatography has emerged as a valuable alternative approach to ion-pair chromatography for the separation of aminoglycoside antibiotics in recent years. however, the resolution of structurally related aminoglycosides is a great challenge owing to the limited selectivity. in this work, a cysteine-based zwitterionic stationary phase (named click te-cys) was utilized and compared with five commonly used hydrophilic interaction liquid chromatography columns. click te-cys displayed much better selectivity for structurally similar aminoglycosides. the retention behaviors of aminoglycosides were investigated in detail, revealing that low ph (2.7 or 3.0) and high buffer concentration (>= 50 mm) were preferable for achieving good peak shape and selectivity. effective resolution of ten aminoglycosides including spectinomycin, dihydrostreptomycin, streptomycin, gentamicin c1, gentamicin c2/c2a, gentamicin c1a, kanamycin, paromonycin, tobramycin, and neomycin was realized at optimized conditions. additionally, spectinomycin and its related impurities were successfully resolved. the results indicated the great potential of the click te-cys column in the separation of aminoglycoside mixtures and related impurities. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem-mass-spectrometry ; hydrophilic interaction chromatography ; performance liquid-chromatography ; light-scattering detection ; stationary-phase ; confirmatory method ; hplc-elsd ; residues ; dihydrostreptomycin ; streptomycin |
| 收录类别 | SCI |
| 资助信息 | 2，2 |
| 原文出处 | 1787 |
| 语种 | 英语 |
| WOS记录号 | WOS:000339669700009 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119711]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 3.Dalian Univ Technol, Chem Anal & Res Ctr, Fac Chem Environm & Biol Sci & Technol, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Jie,Shen, Aijin,Wan, Huihui,et al. Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column[J]. journal of separation science,2014,37(14):1781-1787. |
| APA | Wei, Jie.,Shen, Aijin.,Wan, Huihui.,Yan, Jingyu.,Yang, Bingcheng.,...&Liang, Xinmiao.(2014).Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column.journal of separation science,37(14),1781-1787. |
| MLA | Wei, Jie,et al."Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column".journal of separation science 37.14(2014):1781-1787. |
入库方式： OAI收割
来源：大连化学物理研究所
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