Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides
文献类型：期刊论文
| 作者 | Huang, Guang1,2,3; Xiong, Zhichao1,4; Qin, Hongqiang1; Zhu, Jun1,2; Sun, Zhen1,2; Zhang, Yi1,2; Peng, Xiaojun3; Ou, Junjie1; Zou, Hanfa1 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2014-01-27 |
| 卷号 | 809页码:61-68 |
| 关键词 | Controlled polymerization Hydrophilic interaction chromatography Silica nanoparticles Glycopeptide enrichment Glycoproteomics |
| ISSN号 | 0003-2670 |
| 产权排序 | 待补充 |
| 通讯作者 | 欧俊杰 ; 邹汉法 |
| 合作状况 | 英 |
| 英文摘要 | zwitterionic hydrophilic interaction chromatography (zic-hilic) materials have been increasingly attractive in glycopeptide enrichment. however, the traditional zic-hilic materials are modified with monolayer zwitterionic molecules on the surface, therefore, the hydrophilicity, detection sensitivity and loading capacity are limited. in this work, we synthesized novel silica nanoparticles with uniform poly(2( methacryloyloxy) ethyl) dimethyl-(3-sul-fopropyl) ammonium hydroxide (pmsa) brushes grafted onto the surface via reversible addition-fragmentation chain transfer (raft) polymerization (denoted as sio2-raft@ pmsa). the resulting sio2-raft@ pmsa nanoparticles demonstrated low detection limit (10 fmol) and high recovery yield (over 88%) for glycopeptide enrichment from tryptic digest of human igg. the sio2-raft@ pmsa nanoparticles were further applied for the analysis of mouse liver glycoproteome, a total number of 303 unique n-glycosylation sites corresponding to 185 glycoproteins was reliably profiled in three replicate nano-lc-ms/ms runs. significantly, more glycopeptides were identified than those of nanoparticles, monolayer msa molecules modified sio2@single-msa and nonuniform multilayer pmsa brushes coated sio2@pmsa, as well as commercial zic@hilic beads and click maltose beads. the excellent performance of sio2-raft@pmsa nanoparticles results from the non-fouling property, a large quantity of functional molecules and suitable link arms provided by uniform pmsa brushes, as well as efficient interaction between glycopeptides and uniform pmsa brushes. it is concluded that the synthesized sio2-raft@pmsa nanoparticles exhibit great potential in glycoproteome analysis. moreover, this strategy to modify nanopaticles with uniform polymer brushes via raft polymerization can also be explored to design other types of materials for bioseparation application. crown copyright (c) 2013 published by elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | hydrophilic interaction chromatography ; interaction liquid-chromatography ; fragmentation chain transfer ; n-glycosylated proteins ; mass-spectrometry ; radical polymerization ; selective enrichment ; facile synthesis ; linked glycopeptides ; acid |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 68 |
| 语种 | 英语 |
| WOS记录号 | WOS:000329580500008 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119713]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China 4.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 20023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Guang,Xiong, Zhichao,Qin, Hongqiang,et al. Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides[J]. analytica chimica acta,2014,809:61-68. |
| APA | Huang, Guang.,Xiong, Zhichao.,Qin, Hongqiang.,Zhu, Jun.,Sun, Zhen.,...&Zou, Hanfa.(2014).Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides.analytica chimica acta,809,61-68. |
| MLA | Huang, Guang,et al."Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides".analytica chimica acta 809(2014):61-68. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。