Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins
文献类型：期刊论文
| 作者 | Sun, Zhen1; Dong, Jiaqiang2,3; Zhang, Song2,3; Hu, Zhengyan1; Cheng, Kai1; Li, Kai2,3; Xu, Bo1; Ye, Mingliang1; Nie, Yongzhan2,3; Fan, Daiming2,3 |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 13期号:3页码:1593-1601 |
| 关键词 | quantification glycoprotein chemoresistance leukemia CSC SWATH MS |
| ISSN号 | 1535-3893 |
| 产权排序 | 待补充 |
| 通讯作者 | daiming fan ; 邹汉法 |
| 合作状况 | 英 |
| 英文摘要 | chemoresistance remains the most significant obstacle to successful chemotherapy for leukemia, and its exact mechanism is still unknown. in this work, we used the cell-surface capturing method together with quantitative proteomics to investigate differences in the glycoproteomes of adriamycin-sensitive and adriamycin-resistant leukemia cells. two quantitative methods, isotopic dimethyl labeling and swath, were used to quantify glycoproteins, and 35 glycoproteins were quantified by both methods. high correlation was observed between the glycoproteins quantified by the above two methods, and 15 glycoproteins displayed a consistent significant change trend in both sets of quantitative results. these 15 proteins included classical multidrug resistance-related glycoproteins such as abcb1 as well as a set of novel glycoproteins that have not previously been reported to be associated with chemoresistance in leukemia cells. further validation with quantitative real-time pcr and western blotting confirmed the proteomic screening results. subsequent functional experiments based on rna interference technology showed that ctsd, fkbp10, and slc2a1 are novel genes that participate in the acquisition and maintenance of the adriamycin-resistant phenotype in leukemia cells. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | multidrug-resistance ; cathepsin-d ; mass-spectrometry ; cancer-cells ; n-glycosylation ; protein ; proteomics ; expression ; quantification ; contributes |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 1601 |
| 语种 | 英语 |
| WOS记录号 | WOS:000332756300038 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119728]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi Provinc, Peoples R China 3.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Provinc, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Zhen,Dong, Jiaqiang,Zhang, Song,et al. Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins[J]. journal of proteome research,2014,13(3):1593-1601. |
| APA | Sun, Zhen.,Dong, Jiaqiang.,Zhang, Song.,Hu, Zhengyan.,Cheng, Kai.,...&Zou, Hanfa.(2014).Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins.journal of proteome research,13(3),1593-1601. |
| MLA | Sun, Zhen,et al."Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins".journal of proteome research 13.3(2014):1593-1601. |
入库方式： OAI收割
来源：大连化学物理研究所
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