Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation
文献类型：期刊论文
| 作者 | Xu, Bo1; Wang, Fangjun1; Song, Chunxia1; Sun, Zhen1; Cheng, Kai1; Tan, Yexiong2; Wang, Hongyang2; Zou, Hanfa1 |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 13期号:8页码:3645-3654 |
| 关键词 | Human liver hepatocellular carcinoma proteome and phosphoproteome quantification three-dimensional liquid chromatography separation |
| ISSN号 | 1535-3893 |
| 产权排序 | 待补充 |
| 通讯作者 | 王方军 ; 邹汉法 |
| 合作状况 | 英 |
| 英文摘要 | hepatocellular carcinoma is one of the most fatal cancers worldwide. in this study, a reversed-phase strong cation exchange reversed-phase three-dimensional liquid chromatography strategy was established and coupled with mass spectrometry to investigate the differential proteome expression of hcc and normal liver tissues. in total, 2759 proteins were reliably quantified, of which, 648 proteins were dysregulated more than 3-fold in hcc liver tissues. some important proteins that relate to hcc pathology were significantly dysregulated, such as nat2 and akr1b10. furthermore, 2307 phosphorylation sites from 1264 phosphoproteins were obtained in our previous phosphoproteome quantification, and the non-phosphorylated counterparts of 445 phosphoproteins with 983 phosphorylation sites were reliably quantified in this work. it was observed that 337 (34%) phosphorylation sites exhibit significantly different expression trends from that of their corresponding of hcc were nonphosphoproteins. some novel phosphorylation sites with important biological functions in the progression reliably quantified, such as the significant downregulation of pt185 for erk2 and py204 for erk1. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | phosphoproteome analysis ; quantitative proteomics ; expression ; cancer ; disease ; cell ; identification ; throughput ; separation ; pathways |
| 收录类别 | SCI |
| 资助信息 | 1,1 |
| 原文出处 | 3654 |
| 语种 | 英语 |
| WOS记录号 | WOS:000339983600014 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119767]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Bo,Wang, Fangjun,Song, Chunxia,et al. Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation[J]. journal of proteome research,2014,13(8):3645-3654. |
| APA | Xu, Bo.,Wang, Fangjun.,Song, Chunxia.,Sun, Zhen.,Cheng, Kai.,...&Zou, Hanfa.(2014).Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation.journal of proteome research,13(8),3645-3654. |
| MLA | Xu, Bo,et al."Large-Scale Proteome Quantification of Hepatocellular Carcinoma Tissues by a Three-Dimensional Liquid Chromatography Strategy Integrated with Sample Preparation".journal of proteome research 13.8(2014):3645-3654. |
入库方式： OAI收割
来源：大连化学物理研究所
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