Hydrophilic interaction chromatography for selective separation of isomeric saponins
文献类型：期刊论文
| 作者 | Guo, Xiujie; Zhang, Xiuli; Guo, Zhimou; Liu, Yanfang; Shen, Aijin; Jin, Gaowa; Liang, Xinmiao |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2014-01-17 |
| 卷号 | 1325页码:121-128 |
| 关键词 | Hydrophilic interaction chromatography Methanol Saponins Isomer Separation Panax notoginseng(Burk.) FHChen |
| ISSN号 | 0021-9673 |
| 产权排序 | 待补充 |
| 通讯作者 | 张秀莉 |
| 合作状况 | 英 |
| 英文摘要 | a method based on a click xlon zwitterionic stationary phase was developed to separate isomeric saponins efficiently in hydrophilic interaction chromatography (hilic) mode using methanol as a weak eluent. the retention times of a set of isomeric saponins, ginsenoside rc (s1), ginsenoside rb-2 (s2), and ginsenoside rb-3 (s3) on five kinds of hilic columns were compared using acetonitrile/water or methanol/water as mobile phase. all results indicated that the click xlon column showed the highest retention times of all these isomeric saponins. then the retention behaviors of these isomeric saponins on the click xlon column were investigated. the results demonstrated that the retention behaviors of saponins on click xlon in methanol/water mobile phase were different from that in acetonitrile/water. methanol/water binary eluent offered enhanced selectivity to these isomeric saponins (alpha(s2/s3(meoh)) = 1.16> alpha(s3/s2(acn)) = 1.04), and the elution order of ginsenoside rb-2 (s2) and ginsenoside rb-3 (s3) was reversed compared with acetonitrile/water. moreover, methanol/water solvent has better solubility for saponins than acetonitrile/water, resulting in improved preparative peak shape, which is greatly beneficial for saponin purification. application to the preparative separation of saponins from leaves of p. notoginseng was also investigated, and eleven saponins, including three sets of isomeric saponins with one new saponin were isolated and identified. all these results indicated that this method was efficient for analytical and preparative separation of saponins, especially for isomeric saponins, containing xylopyranosyl, arabinofuranosyl or arabinopyranosyl units. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | interaction liquid-chromatography ; bonded stationary-phase ; multiple linear-regression ; artificial neural-network ; flight mass-spectrometry ; panax-notoginseng ; dammarane-saponins ; subambient temperatures ; plant saponins ; ginsenosides |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 128 |
| 语种 | 英语 |
| WOS记录号 | WOS:000330256200016 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119795]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xiujie,Zhang, Xiuli,Guo, Zhimou,et al. Hydrophilic interaction chromatography for selective separation of isomeric saponins[J]. journal of chromatography a,2014,1325:121-128. |
| APA | Guo, Xiujie.,Zhang, Xiuli.,Guo, Zhimou.,Liu, Yanfang.,Shen, Aijin.,...&Liang, Xinmiao.(2014).Hydrophilic interaction chromatography for selective separation of isomeric saponins.journal of chromatography a,1325,121-128. |
| MLA | Guo, Xiujie,et al."Hydrophilic interaction chromatography for selective separation of isomeric saponins".journal of chromatography a 1325(2014):121-128. |
入库方式： OAI收割
来源：大连化学物理研究所
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