Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides
文献类型：期刊论文
| 作者 | Xiong, Zhichao1,2; Zhang, Lingyi1; Fang, Chunli2; Zhang, Quanqing2; Ji, Yongsheng2,3; Zhang, Zhang2; Zhang, Weibing1,2; Zou, Hanfa2 |
| 刊名 | journal of materials chemistry b
 |
| 出版日期 | 2014 |
| 卷号 | 2期号:28页码:4473-4480 |
| ISSN号 | 0959-9428 |
| 产权排序 | 待补充 |
| 通讯作者 | 张维冰 ; 邹汉法 |
| 合作状况 | 英 |
| 英文摘要 | highly selective and efficient enrichment of trace phosphorylated proteins or peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. in this study, a novel immobilized metal affinity chromatography (imac) material has been synthesized to improve the enrichment specificity and sensitivity for phosphopeptides by introducing a titanium phosphate moiety on a multilayer polysaccharide (hyaluronate (ha) and chitosan (cs)) coated fe3o4@sio2 nanoparticle (denoted as fe3o4@ sio2@(ha/cs)(10)-ti4+ imac). the thicker multilayer polysaccharide endows excellent hydrophilic properties and a higher binding capacity of the titanium ion to the imac material. due to the combination of uniform magnetic properties, highly hydrophilic properties and enhanced binding capacity of the titanium ion, the fe3o4@sio2@(ha/cs)(10)-ti4+ nanoparticle possesses many merits, such as high selectivity for phosphopeptides (phosphopeptides/non-phosphopeptides at a molar ratio of 1 : 2000), extreme detection sensitivity (0.5 fmol), large binding capacity (100 mg g(-1)) high enrichment recovery (85.45%) and rapid magnetic separation (within 10 s). moreover, the as- prepared imac nanoparticle provides effective enrichment of phosphopeptides from real samples (human serum and nonfat milk), showing great potential as a tool for the detection and identification of low-abundance phosphopeptides in biological samples. |
| WOS标题词 | science & technology ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | materials science, biomaterials |
| 研究领域[WOS] | materials science |
| 关键词[WOS] | ion affinity-chromatography ; immobilized metal-ions ; maldi-tof ms ; phosphoproteome analysis ; nanocrystal clusters ; sample preparation ; mass-spectrometry ; in-vivo ; microspheres ; proteins |
| 收录类别 | SCI |
| 资助信息 | 2，1 |
| 原文出处 | 7780 |
| 语种 | 英语 |
| WOS记录号 | WOS:000338646000010 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119821]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 1160237, Peoples R China 3.Henan Univ Tradit Chinese Med, Coll Pharm, Zhengzhou 450000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, Zhichao,Zhang, Lingyi,Fang, Chunli,et al. Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides[J]. journal of materials chemistry b,2014,2(28):4473-4480. |
| APA | Xiong, Zhichao.,Zhang, Lingyi.,Fang, Chunli.,Zhang, Quanqing.,Ji, Yongsheng.,...&Zou, Hanfa.(2014).Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides.journal of materials chemistry b,2(28),4473-4480. |
| MLA | Xiong, Zhichao,et al."Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides".journal of materials chemistry b 2.28(2014):4473-4480. |
入库方式： OAI收割
来源：大连化学物理研究所
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