Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass
文献类型：期刊论文
| 作者 | Li, Yun1,2; Qi, Benkun1; Wan, Yinhua1 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 167期号:sep页码:324-330 |
| 关键词 | Vanillin Cellulase Inhibition kinetics Inhibitory group |
| ISSN号 | 0960-8524 |
| 其他题名 | Bioresour. Technol. |
| 中文摘要 | Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase. (C) 2014 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | ENZYMATIC-HYDROLYSIS ; LIGNOCELLULOSIC BIOMASS ; SACCHAROMYCES-CEREVISIAE ; DEGRADATION-PRODUCTS ; PHENOLIC-COMPOUNDS ; HCH-1 MODEL ; LIGNIN ; PRETREATMENT ; DETOXIFICATION ; FERMENTATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000340891500045 |
| 公开日期 | 2014-09-30 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11456]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yun,Qi, Benkun,Wan, Yinhua. Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass[J]. BIORESOURCE TECHNOLOGY,2014,167(sep):324-330. |
| APA | Li, Yun,Qi, Benkun,&Wan, Yinhua.(2014).Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass.BIORESOURCE TECHNOLOGY,167(sep),324-330. |
| MLA | Li, Yun,et al."Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass".BIORESOURCE TECHNOLOGY 167.sep(2014):324-330. |
入库方式： OAI收割
来源：过程工程研究所
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