Self-assembling doxorubicin-prodrug nanoparticles as siRNA drug delivery system for cancer treatment: in vitro and in vivo
文献类型：期刊论文
| 作者 | Liu, Hongmei1,2; Qiao, Chenmeng3; Yang, Jun1; Weng, Jie3; Zhang, Xin1 |
| 刊名 | JOURNAL OF MATERIALS CHEMISTRY B
 |
| 出版日期 | 2014-09-21 |
| 卷号 | 2期号:35页码:5910-5924 |
| 关键词 | TARGETED CO-DELIVERY POLO-LIKE KINASES POLYMERIC MICELLES ANTICANCER DRUGS THERAPY CELLS GENE CHEMOTHERAPY DAUNOMYCIN NANOCARRIERS |
| ISSN号 | 2050-750X |
| 其他题名 | J. Mat. Chem. B |
| 中文摘要 | Co-delivery of siRNAs and chemotherapeutic drugs to kill tumors have achieved superior tumor growth inhibition. However, due to siRNAs and chemotherapeutic drugs with different molecular properties, co-delivery carriers use more cationic materials to bind siRNAs and excessive inert materials to embed drugs, causing low drug-loading contents and systemic toxicity. To achieve this goal, doxorubicin (DOX) is chemically conjugated to stearoyl chloride (C18) through N-methyldiethanol amine (N) as cross-linker to form amphiphilic C18-N-DOX. C18-N-DOX contains a tertiary amine that can complex siRNAs at low pH (pH 3) and reduce the density of the positive charges on the surface of NPs at physiological pH (pH 7.4). C18-N-DOX, together with 1,2-distearoyl-sn-g/ycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000) DSPE-PEG2000, self-assemble into DOX-prodrug nanoparticles (DOX-prodrug NPs), which bind siRNAs in citrate buffer (pH 3) to form DOX-prodrug NPs/siRNA. After replacing citrate buffer (pH 3) with PBS (pH 7.4), DOX-prodrug NPs/siRNA have slight negative charges due to complexed more siRNAs. In this study, clear evidence is shown that DOX-prodrug NPs can deliver siRNA to the same tumor cells both in vitro and in vivo. Moreover, DOX-prodrug NPs/siRNA show a great effect on inhibiting tumor cell growth both in vitro and in vivo. Therefore, the DOX-prodrug NPs are promising candidates as siRNAs delivery system for tumor therapy. |
| 英文摘要 | Co-delivery of siRNAs and chemotherapeutic drugs to kill tumors have achieved superior tumor growth inhibition. However, due to siRNAs and chemotherapeutic drugs with different molecular properties, co-delivery carriers use more cationic materials to bind siRNAs and excessive inert materials to embed drugs, causing low drug-loading contents and systemic toxicity. To achieve this goal, doxorubicin (DOX) is chemically conjugated to stearoyl chloride (C18) through N-methyldiethanol amine (N) as cross-linker to form amphiphilic C18-N-DOX. C18-N-DOX contains a tertiary amine that can complex siRNAs at low pH (pH 3) and reduce the density of the positive charges on the surface of NPs at physiological pH (pH 7.4). C18-N-DOX, together with 1,2-distearoyl-sn-g/ycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000) DSPE-PEG2000, self-assemble into DOX-prodrug nanoparticles (DOX-prodrug NPs), which bind siRNAs in citrate buffer (pH 3) to form DOX-prodrug NPs/siRNA. After replacing citrate buffer (pH 3) with PBS (pH 7.4), DOX-prodrug NPs/siRNA have slight negative charges due to complexed more siRNAs. In this study, clear evidence is shown that DOX-prodrug NPs can deliver siRNA to the same tumor cells both in vitro and in vivo. Moreover, DOX-prodrug NPs/siRNA show a great effect on inhibiting tumor cell growth both in vitro and in vivo. Therefore, the DOX-prodrug NPs are promising candidates as siRNAs delivery system for tumor therapy. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Materials Science, Biomaterials |
| 研究领域[WOS] | Materials Science |
| 关键词[WOS] | TARGETED CO-DELIVERY ; POLO-LIKE KINASES ; POLYMERIC MICELLES ; ANTICANCER DRUGS ; THERAPY ; CELLS ; GENE ; CHEMOTHERAPY ; DAUNOMYCIN ; NANOCARRIERS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000341274300020 |
| 公开日期 | 2014-09-30 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11468]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Southwest Jiaotong Univ, Sch Mat Sci & Engn, Key Lab Adv Technol Mat, Chengdu 610031, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hongmei,Qiao, Chenmeng,Yang, Jun,et al. Self-assembling doxorubicin-prodrug nanoparticles as siRNA drug delivery system for cancer treatment: in vitro and in vivo[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(35):5910-5924. |
| APA | Liu, Hongmei,Qiao, Chenmeng,Yang, Jun,Weng, Jie,&Zhang, Xin.(2014).Self-assembling doxorubicin-prodrug nanoparticles as siRNA drug delivery system for cancer treatment: in vitro and in vivo.JOURNAL OF MATERIALS CHEMISTRY B,2(35),5910-5924. |
| MLA | Liu, Hongmei,et al."Self-assembling doxorubicin-prodrug nanoparticles as siRNA drug delivery system for cancer treatment: in vitro and in vivo".JOURNAL OF MATERIALS CHEMISTRY B 2.35(2014):5910-5924. |
入库方式： OAI收割
来源：过程工程研究所
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