Effects of cryopreservation on human mesenchymal stem cells attached to different substrates
文献类型：期刊论文
| 作者 | Xu, Xia1,2; Liu, Yang2,3,4; Cui, Zhan Feng2 |
| 刊名 | JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 8期号:8页码:664-672 |
| 关键词 | human mesenchymal stem cells tissue engineering cell attachment cryopreservation cell recovery scaffold design |
| ISSN号 | 1932-6254 |
| 其他题名 | J. Tissue Eng. Regen. Med. |
| 中文摘要 | There is a need to preserve cell-seeded scaffolds or cell-matrix constructs for tissue-engineering and other applications. Cryopreservation is likely to be the most practical method. The aim of this study was to investigate how cryopreservation affects cells attached to different substrates and how they respond differently from those in suspension. Human mesenchymal stem cells (hMSCs) were studied for their close relevance to tissue-engineering and stem cell therapy applications, in particular how cryopreservation affects cell adherence, cell growth and the viability of hMSCs attached to different substrates, including glass, gelatin, matrigel and a matrigel sandwich. The effects of cryopreservation on F-actin organization, intracellular pH and mitochondrial localization of the adherent hMSCs were further investigated. It was found that cells attached to a glass surface could hardly survive the common cryopreservation protocol using 10% DMSO and a 1 degrees C/min cooling rate. By contrast, cells attached to gelatin and matrigel could survive to a greater extent. Furthermore, cryopreservation affected the potential of cell attachment and proliferation, resulted in distortion of F-actin, led to alteration of intracellular pH of the hMSCs for all tested substrates and caused a change in the mitochondrial localization of hMSCs on a matrigel substrate and in a matrigel sandwich. Our results showed that cell attachment and cell viability could be improved by changing the interaction between cell and substrate through modification of the substrate properties, which has implications for scaffold design if cell-seeded scaffolds or engineered tissues need to be cryopreserved. Copyright (C) 2012 John Wiley & Sons, Ltd. |
| 英文摘要 | There is a need to preserve cell-seeded scaffolds or cell-matrix constructs for tissue-engineering and other applications. Cryopreservation is likely to be the most practical method. The aim of this study was to investigate how cryopreservation affects cells attached to different substrates and how they respond differently from those in suspension. Human mesenchymal stem cells (hMSCs) were studied for their close relevance to tissue-engineering and stem cell therapy applications, in particular how cryopreservation affects cell adherence, cell growth and the viability of hMSCs attached to different substrates, including glass, gelatin, matrigel and a matrigel sandwich. The effects of cryopreservation on F-actin organization, intracellular pH and mitochondrial localization of the adherent hMSCs were further investigated. It was found that cells attached to a glass surface could hardly survive the common cryopreservation protocol using 10% DMSO and a 1 degrees C/min cooling rate. By contrast, cells attached to gelatin and matrigel could survive to a greater extent. Furthermore, cryopreservation affected the potential of cell attachment and proliferation, resulted in distortion of F-actin, led to alteration of intracellular pH of the hMSCs for all tested substrates and caused a change in the mitochondrial localization of hMSCs on a matrigel substrate and in a matrigel sandwich. Our results showed that cell attachment and cell viability could be improved by changing the interaction between cell and substrate through modification of the substrate properties, which has implications for scaffold design if cell-seeded scaffolds or engineered tissues need to be cryopreserved. Copyright (C) 2012 John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology ; Engineering, Biomedical |
| 研究领域[WOS] | Cell Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | BONE-MARROW ; IN-VITRO ; CULTURE ; MATRIX ; PROLIFERATION ; HEPATOCYTES ; PROGENITORS ; MONOLAYERS ; REMOVAL ; COMPLEX |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000340245900009 |
| 公开日期 | 2014-09-30 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11578]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 2.Univ Oxford, Dept Engn Sci, Inst Biomed Engn, Oxford OX1 3PJ, England 3.Dalian Univ Technol, Sch Chem Engn, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian, Peoples R China 4.Dalian Med Univ, Regenerat Med Ctr, Affiliated Hosp 1, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Xia,Liu, Yang,Cui, Zhan Feng. Effects of cryopreservation on human mesenchymal stem cells attached to different substrates[J]. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2014,8(8):664-672. |
| APA | Xu, Xia,Liu, Yang,&Cui, Zhan Feng.(2014).Effects of cryopreservation on human mesenchymal stem cells attached to different substrates.JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,8(8),664-672. |
| MLA | Xu, Xia,et al."Effects of cryopreservation on human mesenchymal stem cells attached to different substrates".JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 8.8(2014):664-672. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。