Effects of steroid hormones on reproduction- and detoxification-related gene expression in adult male mosquitofish, Gambusia affinis
文献类型：期刊论文
| 作者 | Huang, Guo-Yong; Ying, Guang-Guo; Liang, Yan-Qiu; Liu, You-Sheng; Liu, Shuang-Shuang |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
 |
| 出版日期 | 2013-05-31 |
| 卷号 | 158期号:1页码:36-43 |
| 关键词 | Cytochrome P4501A Endocrine disruption Hormone receptor Metallothionein Vitellogenin |
| ISSN号 | 1532-0456 |
| 英文摘要 | The molecular mechanisms that mediate fish reproduction and detoxification in response to steroid hormones were studied by using adult male western mosquitofish (Gambusia affinis) as sentinel species. The expression patterns of three vitellogenins (VtgA, VtgB and VtgC), two estrogen receptors (ER alpha and ER beta), two androgen receptors (AR alpha and AR beta), metallothionein (MT) and cytochrome P450 1A (CYP1A) in the liver and testis of adult male mosquitofish were assessed through exposure treatments with progesterone (P), testosterone (T) and 17 beta-estradiol (E2), alone and in combination for eight days. The results showed that expression patterns of Vtg subtype, ER subtype, AR subtype, MT and CYPIA genes in male mosquitofish varied according to tissue and specific hormone stress. Vtg subtype mRNA expression was induced in the liver in E2-added treatments, and an up-regulation of ER alpha mRNA expression was also observed. In addition, hormone treatments increased three Vtg subtype mRNA expression levels in the testis, at least to some extent. All hormone treatments significantly inhibited ER alpha, ER beta and AR beta mRNA expression in the testis. Some of hormone treatments could affect MT and CYP1A gene expression in mosquitofish. In general, multiple hormone treatments showed different effects on target gene expression compared with corresponding hormone alone. The results from the present study provided valuable information on the toxicological effects of steroid hormones in mosquitofish. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| 关键词[WOS] | ENDOCRINE-DISRUPTING CHEMICALS ; RAINBOW-TROUT HEPATOCYTES ; BASS DICENTRARCHUS-LABRAX ; ZEBRAFISH DANIO-RERIO ; ESTROGEN-RECEPTOR ; DIFFERENTIAL EXPRESSION ; VITELLOGENIN GENES ; ANDROGEN RECEPTOR ; IN-VITRO ; 17-BETA-ESTRADIOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000320746400005 |
| 公开日期 | 2014-10-14 |
| 源URL | [http://ir.gig.ac.cn:8080/handle/344008/19136]  |
| 专题 | 中国科学院广州地球化学研究所 |
| 作者单位 | Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Guo-Yong,Ying, Guang-Guo,Liang, Yan-Qiu,et al. Effects of steroid hormones on reproduction- and detoxification-related gene expression in adult male mosquitofish, Gambusia affinis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2013,158(1):36-43. |
| APA | Huang, Guo-Yong,Ying, Guang-Guo,Liang, Yan-Qiu,Liu, You-Sheng,&Liu, Shuang-Shuang.(2013).Effects of steroid hormones on reproduction- and detoxification-related gene expression in adult male mosquitofish, Gambusia affinis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,158(1),36-43. |
| MLA | Huang, Guo-Yong,et al."Effects of steroid hormones on reproduction- and detoxification-related gene expression in adult male mosquitofish, Gambusia affinis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 158.1(2013):36-43. |
入库方式： OAI收割
来源：广州地球化学研究所
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