Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways
文献类型：期刊论文
| 作者 | Li, L.1,2,3 ; Yue, G. G. L.2,3; Pu, J. X.4; Sun, H. D.4; Fung, K. P.1,2,3; Leung, P. C.2,3; Han, Q. B.5; Lau, C. B. S.2,3; Leung, P. S.1
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| 刊名 | CURRENT MOLECULAR MEDICINE
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| 出版日期 | 2014 |
| 卷号 | 14期号:5页码:673-689 |
| 关键词 | Apoptosis eriocalyxin B pancreatic cancer reactive oxygen species thiols |
| ISSN号 | 1566-5240 |
| 产权排序 | 第一 |
| 通讯作者 | Xu, JC (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China. ; j.c.xu@cgiar.org ; kdhyde3@gmail.com |
| 英文摘要 | Thiol-containing antioxidant systems play an important role in regulating cellular redox homeostasis. Several anti-cancer agents act by targeting these systems by inducing the production of reactive oxygen species (ROS). Our earlier studies have shown that Eriocalyxin B (EriB), a diterpenoid isolated from Isodon eriocalyx, possesses anti-pancreatic tumour activities in vitro and in vivo. The present study further demonstrated that only thiol-containing antioxidants, N-acetylcysteine (NAC) or dithiothreitol (DTT), inhibited EriB-induced cytotoxicity and apoptosis. EriB suppressed the glutathione and thioredoxin antioxidant systems, thus increasing the intracellular ROS levels and regulating the MAPK, NF kappa B pathways. Treatment with EriB depleted the intracellular thiol-containing proteins in CAPAN-2 cells. In vivo studies also showed that EriB treatment (2.5 mg/kg) reduced the pancreatic tumour weights significantly in nude mice with increased superoxide levels. Taken together, our results shed important new light on the molecular mechanisms of EriB acting as an apoptogenic agent and its therapeutic potential for pancreatic cancer. |
| 学科主题 | Medicine, Research & Experimental |
| 类目[WOS] | Medicine, Research & Experimental |
| 研究领域[WOS] | Research & Experimental Medicine |
| 关键词[WOS] | FACTOR-KAPPA-B ; OXIDATIVE STRESS ; THIOREDOXIN REDUCTASE ; CANCER CELLS ; MAMMALIAN THIOREDOXIN ; REDOX REGULATION ; TUMOR-GROWTH ; IN-VITRO ; INHIBITION ; GLUTATHIONE |
| 收录类别 | SCI |
| 资助信息 | Health and Medical Research Fund of Food and Health Bureau, Hong Kong Special Administrative Region [10110021] |
| 语种 | 英语 |
| WOS记录号 | WOS:000338528100009 |
| 公开日期 | 2014-10-11 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/18277]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Univ Hong Kong, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China 2.Chinese Univ Hong Kong, Inst Chinese Med, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Hong Kong, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Mengla, Yunnan, Peoples R China 5.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, L.,Yue, G. G. L.,Pu, J. X.,et al. Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways[J]. CURRENT MOLECULAR MEDICINE,2014,14(5):673-689. |
| APA | Li, L..,Yue, G. G. L..,Pu, J. X..,Sun, H. D..,Fung, K. P..,...&Leung, P. S..(2014).Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways.CURRENT MOLECULAR MEDICINE,14(5),673-689. |
| MLA | Li, L.,et al."Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways".CURRENT MOLECULAR MEDICINE 14.5(2014):673-689. |
入库方式： OAI收割
来源：昆明植物研究所
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