Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization
文献类型：期刊论文
| 作者 | Dai GL(戴国亮) ; Zheng, L; Sazaki, G; Furukawa, Y
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| 刊名 | CRYSTAL GROWTH & DESIGN
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| 出版日期 | 2014-10 |
| 卷号 | 14期号:10页码:5303-5309 |
| 通讯作者邮箱 | sazaki@lowtem.hokudai.ac.jp |
| ISSN号 | 1528-7483 |
| 产权排序 | [Dai, Guoliang] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Dai, Guoliang; Sazaki, Gen; Furukawa, Yoshinori] Hokkaido Univ, Inst Low Temp Sci, Kita Ku, Sapporo, Hokkaido 0600819, Japan; [Zheng, Lei] Southeast Univ, Sch Civil Engn, Nanjing 210096, Jiangsu, Peoples R China |
| 通讯作者 | Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, Kita Ku, N19-W8, Sapporo, Hokkaido 0600819, Japan. |
| 合作状况 | 国际 |
| 中文摘要 | On a {10 (1) over bar} face of a monoclinic crystal of hen egg-white lysozyme (HEWL), we visualized the attachment and detachment processes of individual fluorescent-labeled HEWL (F-HEWL) molecules by a fluorescent single-molecule visualization technique. We measured the changes in number density of F-HEWL molecules, whose positions were not changed for longer than a certain residence time, as a function of an adsorption time. We first confirmed that under an equilibrium condition, there was an induction period (similar to 120 min) of the attachment/detachment processes, during which period the number density remained constant. After the induction period, the number density increased linearly with the adsorption time, as it was recently found on a tetragonal HEWL crystal [Dai, G. L. Cryst. Growth Des 2011, 11 (1), 88-92]. In addition, we performed similar measurements under a supersaturated condition. Then we found that supersaturation significantly enhanced the attachment process after the induction time. The attachment/detachment processes finally reached a steady state, in which the attachment rate was higher than the detachment one. Moreover, we also found that in a rare case, an F-HEWL molecule adsorbed on a step laterally moved following the advancement of a growing step. |
| 分类号 | 一类 |
| 收录类别 | SCI ; EI |
| 资助信息 | G.D. acknowledges the support by National Program on Key Basic Research Project (973 Program) (No. 2011CB710901), and the visiting professorship from Institute of Low Temperature Science, Hokkaido University. G.S. is grateful for the support by Chinese Academy of Sciences visiting professorship for senior international scientists (Grant No. 2013T2J0060). The authors thank Y. Saito and S. Kobayashi (Olympus Engineering Co., Ltd.) for their technical support of LCM-DIM. Funding is from National Program on Key Basic Research Project (973 Program) (No. 2011CB710901); visiting professorship from Institute of Low Temperature Science, Hokkaido University; Chinese Academy of Sciences visiting professorship for senior international scientists (Grant No. 2013T2J0060). |
| 原文出处 | http://dx.doi.org/10.1021/cg501122f |
| 语种 | 英语 |
| WOS记录号 | WOS:000342609300055 |
| 公开日期 | 2014-11-27 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/49346]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Dai GL,Zheng, L,Sazaki, G,et al. Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization[J]. CRYSTAL GROWTH & DESIGN,2014,14(10):5303-5309. |
| APA | Dai GL,Zheng, L,Sazaki, G,&Furukawa, Y.(2014).Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization.CRYSTAL GROWTH & DESIGN,14(10),5303-5309. |
| MLA | Dai GL,et al."Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization".CRYSTAL GROWTH & DESIGN 14.10(2014):5303-5309. |
入库方式： OAI收割
来源：力学研究所
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