Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19)
文献类型：期刊论文
| 作者 | Yang, Lijuan ; Lu, Danfeng ; Guo, Jiajia ; Meng, Xianli ; Zhang, Guolin ; Wang, Fei |
| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 145期号:3页码:715-721 |
| 关键词 | Icariin Estrogen biosynthesis Aromatase Granulosa cell Osteoblast |
| ISSN号 | 0378-8741 |
| 产权排序 | 1 |
| 通讯作者 | Zhang, GL (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China ; Wang, Fei |
| 英文摘要 | Ethnopharmacological relevance: Epimedium brevicornum Maxim has long been used for the treatment of osteoporosis in China and other Asian countries. However, the mechanism behind the antiosteoporotic activity of this medicinal plant is not fully understood. Aim of the study: The present study was designed to investigate the effects of five widely used antiosteoporotic medicinal plants (Epimedium brevicornum, Cuscuta chinensis, Rhizoma drynariae, Polygonum multiflorum, and Ligustrum lucidum) on the production of estrogen, and identify the bioactive compounds responsible for the estrogen biosynthesis-promoting effect. Materials and methods: Human ovarian granulosa-like KGN cells were used to evaluate estrogen biosynthesis, and the production of 17 beta-estradiol was quantified by a magnetic particle-based enzyme-linked immunosorbent assay (ELISA) kit. Further, the mRNA expression of aromatase was determined by a quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR), and the protein expression of aromatase was detected by western blotting. The activity of alkaline phosphatase (ALP) in rat osteoblastic UMR-106 cells was measured using p-nitrophenyl sodium phosphate assay. Results: Among the 5 antiosteoporotic medicinal plants, the extract of Epimedium brevicornum was found to significantly promote estrogen biosynthesis in KGN cells. Icariin, the major compound in Epimedium brevicornum, was identified to be the active compound for the estrogen biosynthesis-promoting effect. Icariin promoted estrogen biosynthesis in KGN cells in a concentration- and time-dependant manner and enhanced the mRNA and protein expressions of aromatase, which is the only enzyme for the conversion of androgens to estrogens in vertebrates. Further study showed that icariin also promoted estrogen biosynthesis and ALP activity in osteoblastic UMR-106 cells. Conclusions: These results show that the promotion of estrogen biosynthesis is a novel effect of Epimedium brevicornum, and icariin could be utilized for the prevention and treatment of osteoporosis. (C) 2012 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315005100005 |
| 公开日期 | 2014-11-21 |
| 源URL | [http://210.75.237.14/handle/351003/24176]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Yang, Lijuan,Lu, Danfeng,Guo, Jiajia,et al. Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19)[J]. JOURNAL OF ETHNOPHARMACOLOGY,2013,145(3):715-721. |
| APA | Yang, Lijuan,Lu, Danfeng,Guo, Jiajia,Meng, Xianli,Zhang, Guolin,&Wang, Fei.(2013).Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19).JOURNAL OF ETHNOPHARMACOLOGY,145(3),715-721. |
| MLA | Yang, Lijuan,et al."Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19)".JOURNAL OF ETHNOPHARMACOLOGY 145.3(2013):715-721. |
入库方式： OAI收割
来源：成都生物研究所
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