Cleavage-based signal amplification of RNA
文献类型：期刊论文
| 作者 | Zhao, Yongyun ; Zhou, Li ; Tang, Zhuo |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2013 |
| 卷号 | 4页码:- |
| ISSN号 | 2041-1723 |
| 产权排序 | 1 |
| 通讯作者 | Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China. |
| 英文摘要 | RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription-PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detect RNA, based on cleavage by DNAzyme and signal amplification. Cleavage-based signal amplification of RNA cannot be contaminated by genomic DNA and is suitable for the detection of both mRNA and microRNA targets, with high specificity and sensitivity. Moreover, the detection results can be reported in a colorimetric or real-time fluorometric way for different detection purposes. |
| 学科主题 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316616400063 |
| 公开日期 | 2014-11-21 |
| 源URL | [http://210.75.237.14/handle/351003/24178]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhao, Yongyun,Zhou, Li,Tang, Zhuo. Cleavage-based signal amplification of RNA[J]. NATURE COMMUNICATIONS,2013,4:-. |
| APA | Zhao, Yongyun,Zhou, Li,&Tang, Zhuo.(2013).Cleavage-based signal amplification of RNA.NATURE COMMUNICATIONS,4,-. |
| MLA | Zhao, Yongyun,et al."Cleavage-based signal amplification of RNA".NATURE COMMUNICATIONS 4(2013):-. |
入库方式： OAI收割
来源：成都生物研究所
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