RNA pathogen detection with one-step reverse transcription PCR and strand-displacement based signal amplification
文献类型：期刊论文
| 作者 | Du, Feng ; Streckenbach, Frank ; Chen, Haodong ; Huang, Xin ; Tang, Zhuo ; Marx, Andreas |
| 刊名 | ANALYST
 |
| 出版日期 | 2013 |
| 卷号 | 138期号:5页码:1544-1548 |
| ISSN号 | 0003-2654 |
| 产权排序 | 1 |
| 通讯作者 | Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China ; Marx, Andreas |
| 英文摘要 | A novel detection method for RNA pathogens based on one-step reverse transcription PCR is introduced here. This method utilized the reverse transcriptase activity and the 5'-nuclease activity of TaqM1 DNA polymerase to transform target RNA into cDNA. The following PCR process released a fragment from the 5' end as a specific probe. Afterwards this fragment triggered a strand-displacement based signal amplification to release large amounts of G-quadruplex DNAzymes. All the probes applied in our method were unmodified DNA oligonucleotides. The detection results could be reported without sophisticated instruments either in the colorimetric way through oxidizing ABTS or in the fluorometric way by using tyramine as substrate. This approach could successfully detect HIV-1 in a blood sample and it has a linear concentration range of 6 fM to 60 pM. |
| 学科主题 | Chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000314473000033 |
| 公开日期 | 2014-11-21 |
| 源URL | [http://210.75.237.14/handle/351003/24192]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Du, Feng,Streckenbach, Frank,Chen, Haodong,et al. RNA pathogen detection with one-step reverse transcription PCR and strand-displacement based signal amplification[J]. ANALYST,2013,138(5):1544-1548. |
| APA | Du, Feng,Streckenbach, Frank,Chen, Haodong,Huang, Xin,Tang, Zhuo,&Marx, Andreas.(2013).RNA pathogen detection with one-step reverse transcription PCR and strand-displacement based signal amplification.ANALYST,138(5),1544-1548. |
| MLA | Du, Feng,et al."RNA pathogen detection with one-step reverse transcription PCR and strand-displacement based signal amplification".ANALYST 138.5(2013):1544-1548. |
入库方式： OAI收割
来源：成都生物研究所
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