Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences
文献类型：期刊论文
| 作者 | Wang, M ; Zhao, HX ; Wang, L ; Wang, T ; Yang, RW ; Wang, XL ; Zhou, YH ; Ding, CB ; Zhang, L |
| 刊名 | GENE
 |
| 出版日期 | 2013 |
| 卷号 | 528期号:2页码:206-215 |
| 关键词 | Salvia DNA barcoding ITS1 trnL–F |
| ISSN号 | 0378-1119 |
| 产权排序 | 3 |
| 通讯作者 | Zhang, L |
| 中文摘要 | An effective DNA marker for authenticating the genus Salvia was screened using seven DNA regions (rbcL, matK, trnlL-F, and psbA-trnH from the chloroplast genome, and ITS, ITS1, and ITS2 from the nuclear genome) and three combinations (rbcL + matK, psbA-trnH + ITS1, and trnL-F + ITS1). The present study collected 232 sequences from 27 Salvia species through DNA sequencing and 77 sequences within the same taxa from the GenBank. The discriminatory capabilities of these regions were evaluated in terms of PCR amplification success, intraspecific and interspecific divergence, DNA barcoding gaps, and identification efficiency via a tree-based method. ITS1 was superior to the other marker for discriminating between species, with an accuracy of 81.48%. The three combinations did not increase species discrimination. Finally, we found that ITS1 is a powerful barcode for identifying Salvia species, especially Salvia miltiorrhiza. |
| 学科主题 | Genetics & Heredity |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324565600019 |
| 公开日期 | 2014-11-21 |
| 源URL | [http://210.75.237.14/handle/351003/24263]  |
| 专题 | 成都生物研究所_生态研究 |
| 推荐引用方式 GB/T 7714 | Wang, M,Zhao, HX,Wang, L,et al. Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences[J]. GENE,2013,528(2):206-215. |
| APA | Wang, M.,Zhao, HX.,Wang, L.,Wang, T.,Yang, RW.,...&Zhang, L.(2013).Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences.GENE,528(2),206-215. |
| MLA | Wang, M,et al."Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences".GENE 528.2(2013):206-215. |
入库方式： OAI收割
来源：成都生物研究所
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