Abundance and community structure of ammonia-oxidizing bacteria and archaea in a temperate forest ecosystem under ten-years elevated CO2
文献类型：期刊论文
| 作者 | Long, Xien ; Chen, Chengrong ; Xu, Zhihong ; Oren, Ram ; He, Ji-Zheng |
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2012 |
| 卷号 | 46页码:163－171 |
| 关键词 | Ammonia-oxidizing bacteria and archaea Elevated CO2 Free air CO2 enrichment (FACE) experiment N fertilization Nitrification Temperate forest ecosystem |
| ISSN号 | 0038-0717 |
| 中文摘要 | Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are considered as the key drivers of global nitrogen (N) biogeochemical cycling. Responses of the associated microorganisms to global changes remain unclear. This study was to determine if there was a shift in soil AOB and AOA abundances and community structures under free-air carbon dioxide (CO2) enrichment (FACE) and N fertilization in Duke Forest of North Carolina, by using DNA-based molecular techniques, i.e., quantitative PCR, restriction fragment length polymorphism (RFLP) and clone library. The N fertilization alone increased the abundance of bacterial amoA gene, but this effect was not observed under elevated CO2 condition. There was no significant effect of the N fertilization on the thaumarchaeal amoA gene abundance in the ambient CO2 treatments, while such effect increased significantly under elevated CO2. A total of 690 positive clones for AOA and 607 for AOB were selected for RFLP analysis. Analysis of molecular variance (AMOVA) indicated that effects of CO2 enrichment and N fertilization on the community structure of AOA and AOB were not significant. Canonical correspondence analysis also showed that soil pH rather than elevated CO2 or N fertilization shaped the distribution of AOB and AOA genotypes. A negative linear relationship between the delta C-13 and archaeal amoA gene abundance indicated a positive effect of elevated CO2 on the growth ammonia oxidizing archaea. On the other hand, the community structures of AOB and AOA are determined by the soil niche properties rather than elevated CO2 and N fertilization. (C) 2011 Elsevier Ltd. All rights reserved. |
| WOS记录号 | WOS:000300743400022 |
| 公开日期 | 2014-11-05 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7854]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Long, Xien,Chen, Chengrong,Xu, Zhihong,et al. Abundance and community structure of ammonia-oxidizing bacteria and archaea in a temperate forest ecosystem under ten-years elevated CO2[J]. SOIL BIOLOGY & BIOCHEMISTRY,2012,46:163－171. |
| APA | Long, Xien,Chen, Chengrong,Xu, Zhihong,Oren, Ram,&He, Ji-Zheng.(2012).Abundance and community structure of ammonia-oxidizing bacteria and archaea in a temperate forest ecosystem under ten-years elevated CO2.SOIL BIOLOGY & BIOCHEMISTRY,46,163－171. |
| MLA | Long, Xien,et al."Abundance and community structure of ammonia-oxidizing bacteria and archaea in a temperate forest ecosystem under ten-years elevated CO2".SOIL BIOLOGY & BIOCHEMISTRY 46(2012):163－171. |
入库方式： OAI收割
来源：生态环境研究中心
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