热门
Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils
文献类型：期刊论文
| 作者 | Zhang, Li-Mei ; Hu, Hang-Wei ; Shen, Ju-Pei ; He, Ji-Zheng |
| 刊名 | ISME JOURNAL
 |
| 出版日期 | 2012 |
| 卷号 | 6期号:5页码:1032－1045 |
| 关键词 | acidic soil ammonia-oxidizing archaea ammonia-oxidizing bacteria dicyandiamide nitrification stable isotope probing |
| ISSN号 | 1751-7362 |
| 中文摘要 | Increasing evidence demonstrated the involvement of ammonia-oxidizing archaea (AOA) in the global nitrogen cycle, but the relative contributions of AOA and ammonia-oxidizing bacteria (AOB) to ammonia oxidation are still in debate. Previous studies suggest that AOA would be more adapted to ammonia-limited oligotrophic conditions, which seems to be favored by protonation of ammonia, turning into ammonium in low-pH environments. Here, we investigated the autotrophic nitrification activity of AOA and AOB in five strongly acidic soils (pH<4.50) during microcosm incubation for 30 days. Significantly positive correlations between nitrate concentration and amoA gene abundance of AOA, but not of AOB, were observed during the active nitrification. (CO2)-C-13-DNA-stable isotope probing results showed significant assimilation of C-13-labeled carbon source into the amoA gene of AOA, but not of AOB, in one of the selected soil samples. High levels of thaumarchaeal amoA gene abundance were observed during the active nitrification, coupled with increasing intensity of two denaturing gradient gel electrophoresis bands for specific thaumarchaeal community. Addition of the nitrification inhibitor dicyandiamide (DCD) completely inhibited the nitrification activity and CO2 fixation by AOA, accompanied by decreasing thaumarchaeal amoA gene abundance. Bacterial amoA gene abundance decreased in all microcosms irrespective of DCD addition, and mostly showed no correlation with nitrate concentrations. Phylogenetic analysis of thaumarchaeal amoA gene and 16S rRNA gene revealed active (CO2)-C-13-labeled AOA belonged to groups 1.1a-associated and 1.1b. Taken together, these results provided strong evidence that AOA have a more important role than AOB in autotrophic ammonia oxidation in strongly acidic soils. The ISME Journal (2012) 6, 1032-1045; doi:10.1038/ismej.2011.168; published online 1 December 2011 |
| WOS记录号 | WOS:000302950700012 |
| 公开日期 | 2014-11-05 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7856]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Li-Mei,Hu, Hang-Wei,Shen, Ju-Pei,et al. Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils[J]. ISME JOURNAL,2012,6(5):1032－1045. |
| APA | Zhang, Li-Mei,Hu, Hang-Wei,Shen, Ju-Pei,&He, Ji-Zheng.(2012).Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils.ISME JOURNAL,6(5),1032－1045. |
| MLA | Zhang, Li-Mei,et al."Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils".ISME JOURNAL 6.5(2012):1032－1045. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。