中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Responses of activities, abundances and community structures of soil denitrifiers to short-term mercury stress

文献类型:期刊论文

作者Zhou, Zhifeng ; Zheng, Yuanming ; Shen, Jupei ; Zhang, Limei ; Liu, Yurong ; He, Jizheng
刊名Environmental Sciences & Ecology
出版日期2012
卷号24期号:3页码:369-375
关键词Hg denitrification enzyme activity nirS nosZ DGGE real-time PCR
ISSN号1001-0742
中文摘要The responses of activities, abundances and community structures of soil denitrifiers to mercury (Hg) stress were investigated through a short-term incubation experiment. Four soil treatments with different concentrations of Hg (CK, Hg25, Hg50, and Hg100, denoted as 0, 25, 50, and 100 mg Hg/kg dry soil, respectively) were incubated for 28 days. Soil denitrification enzyme activity (DEA) was measured at day 3, 7 and 28. The abundances and community structures of two denitrification concerning genes, nirS (cd(1)-nitrite reductase gene) and nosZ (nitrous oxide reductase gene), were analyzed using real-time PCR and denaturing gradient gel electrophoresis (DGGE). Results showed that soil DEA was significantly stimulated in the treatments of Hg25 and Hg50 compared with others at day 7. Meanwhile, no difference in the abundances of soil nirS and nosZ was found between Hg spiked treatments and CK, except the lower abundance of nirS (P < 0.05) in the Hg added treatments compared with that in the CK at day 28. The community structures of denitrifiers based on nirS gene presented obvious change at day 7 along with the Hg additions, however, no variation was found in all treatments based on the nosZ gene. The results indicated that Hg (Hg25 and Hg50) had a strongly short-term stimulation on soil DEA, and nirS gene is more sensitive than nosZ gene to Hg stress.
WOS记录号WOS:000301869300003
公开日期2014-11-26
源URL[http://ir.rcees.ac.cn/handle/311016/7993]  
专题生态环境研究中心_城市与区域生态国家重点实验室
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GB/T 7714
Zhou, Zhifeng,Zheng, Yuanming,Shen, Jupei,et al. Responses of activities, abundances and community structures of soil denitrifiers to short-term mercury stress[J]. Environmental Sciences & Ecology,2012,24(3):369-375.
APA Zhou, Zhifeng,Zheng, Yuanming,Shen, Jupei,Zhang, Limei,Liu, Yurong,&He, Jizheng.(2012).Responses of activities, abundances and community structures of soil denitrifiers to short-term mercury stress.Environmental Sciences & Ecology,24(3),369-375.
MLA Zhou, Zhifeng,et al."Responses of activities, abundances and community structures of soil denitrifiers to short-term mercury stress".Environmental Sciences & Ecology 24.3(2012):369-375.

入库方式: OAI收割

来源:生态环境研究中心

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