An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China
文献类型：期刊论文
| 作者 | Li Xiao-Fang ; Yin Hong-Bin ; Su Jian-Qiang |
| 刊名 | Agriculture
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:2页码:201-205 |
| 关键词 | copA Cu contamination pollution-induced community tolerance (PICT) quantitative PCR |
| ISSN号 | 1002-0160 |
| 中文摘要 | Understanding the mechanisms of Cu pollution-induced community tolerance (PICT) in soil requires the characterization of Cu-resistant microorganisms at a community level using modern molecular tools. A primer pair (copAF2010 (5'-TGCAC CTGAC VGGSC AYAT-3')/copAR2333 (5'-GVACT TCRCG GAACA TRCC-3')) tentatively targeting Pseudomonas-like Cu-resistant microorganisms was designed in this study. The specification of the primers was tested through conventional polymerase chain reaction (PCR) and the construction of a Pseudomonas-like copA gene fragment library, and then the primers were used to quantify the Cu-resistant microorganisms using quantitative PCR technique. A significant increase of Cu-resistant microorganisms targeted by the primers was observed in a paddy soil from Jiaxing, China which has been exposed to one-year Cu contamination. The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution. |
| WOS记录号 | WOS:000302204200006 |
| 公开日期 | 2014-11-26 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/8007]  |
| 专题 | 生态环境研究中心_大气环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Li Xiao-Fang,Yin Hong-Bin,Su Jian-Qiang. An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China[J]. Agriculture,2012,22(2):201-205. |
| APA | Li Xiao-Fang,Yin Hong-Bin,&Su Jian-Qiang.(2012).An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China.Agriculture,22(2),201-205. |
| MLA | Li Xiao-Fang,et al."An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China".Agriculture 22.2(2012):201-205. |
入库方式： OAI收割
来源：生态环境研究中心
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