Electrocatalytic oxidation of tyrosines shows signal enhancement in label-free protein biosensors
文献类型：期刊论文
| 作者 | Wei, Ming-Yuan ; Guo, Liang-Hong ; Famouri, Parviz |
| 刊名 | TRAC-TRENDS IN ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2012 |
| 卷号 | 39页码:130－148 |
| 关键词 | Bioanalytical detection Biosensor Electrocatalytic oxidation Electrochemistry Label-free protein biosensor Osmium bipyridine Phenoxazine Signal enhancement Protein function Tyrosine |
| ISSN号 | 0165-9936 |
| 中文摘要 | Label-free electrochemical (EC) protein biosensors that derive electrical signal from redox-active amino acid (AA) residues can avoid disruption of delicate protein structures, and thus provide a great opportunity to reveal valid information about protein functions. However, the challenge is that such a signal is usually very limited due to the sluggish EC reaction of free AAs on most common electrodes and slow electron-transfer rates from the deeply-buried AA residues in a protein to the electrode. Signal enhancement therefore becomes crucial. We first survey recent progress in this area. We present a signal-enhancing system that relies on the electrocatalytic oxidation of tyrosine mediated by osmium bipyridine or phenoxazine complexes. We describe several applications of label-free protein EC biosensors based on this detection principle for the analysis of protein functions, including the monitoring of protein-conformation change, study of ligand/protein binding, and detection of protein oxidative damage and protein phosphorylation. We describe related works on protein-function analysis using other signal-enhancing methods. The results suggest that label-free EC protein biosensors are suitable for the rapid survey of protein functions due to their fast response, ease of integration, cost effectiveness and convenience. Proof-of-concept work on the application of our system is paving the way for bio-analytical detections and protein-function analysis in future work. (c) 2012 Elsevier Ltd. All rights reserved. |
| WOS记录号 | WOS:000309026300019 |
| 公开日期 | 2014-11-04 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7731]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wei, Ming-Yuan,Guo, Liang-Hong,Famouri, Parviz. Electrocatalytic oxidation of tyrosines shows signal enhancement in label-free protein biosensors[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2012,39:130－148. |
| APA | Wei, Ming-Yuan,Guo, Liang-Hong,&Famouri, Parviz.(2012).Electrocatalytic oxidation of tyrosines shows signal enhancement in label-free protein biosensors.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,39,130－148. |
| MLA | Wei, Ming-Yuan,et al."Electrocatalytic oxidation of tyrosines shows signal enhancement in label-free protein biosensors".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 39(2012):130－148. |
入库方式： OAI收割
来源：生态环境研究中心
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