An E. coli SOS-EGFP biosensor for fast and sensitive detection of DNA damaging agents
文献类型：期刊论文
| 作者 | Chen, Zhilan ; Lu, Meiling ; Zou, Dandan ; Wang, Hailin |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2012 |
| 卷号 | 24期号:3页码:541－549 |
| 关键词 | bacterial biosensor detection DNA damage genotoxins SOS response |
| ISSN号 | 1001-0742 |
| 中文摘要 | An E. coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response was constructed for detection of DNA damage and evaluation of DNA damaging chemicals. The chemicals that may cause substantial DNA damage will trigger SOS response in the constructed bacterial biosensor, and then the reporter egfp gene under the control of recA promoter is stimulated to express as a fluorescent protein, allowing fast and sensitive fluorescence detection. Interestingly, this biosensor can be simultaneously applied for evaluation of genotoxicity and cytotoxicity. The SOS-EGFP bacterial biosensor provides a sensitive, specific and simple method for detecting known and potential DNA damaging chemicals. |
| WOS记录号 | WOS:000301869300026 |
| 公开日期 | 2014-11-04 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7786]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Chen, Zhilan,Lu, Meiling,Zou, Dandan,et al. An E. coli SOS-EGFP biosensor for fast and sensitive detection of DNA damaging agents[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2012,24(3):541－549. |
| APA | Chen, Zhilan,Lu, Meiling,Zou, Dandan,&Wang, Hailin.(2012).An E. coli SOS-EGFP biosensor for fast and sensitive detection of DNA damaging agents.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,24(3),541－549. |
| MLA | Chen, Zhilan,et al."An E. coli SOS-EGFP biosensor for fast and sensitive detection of DNA damaging agents".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 24.3(2012):541－549. |
入库方式： OAI收割
来源：生态环境研究中心
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