Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage
文献类型：期刊论文
| 作者 | Liu, Yang ; Jia, Suping ; Guo, Liang-Hong |
| 刊名 | Chemistry; Electrochemistry; Instruments & Instrumentation
 |
| 出版日期 | 2012 |
| 卷号 | 161期号:1页码:334-340 |
| 关键词 | Microplate Electrode array Tin oxide Photoelectrochemistry DNA damage |
| ISSN号 | 0925-4005 |
| 中文摘要 | Many chemicals have been found to induce DNA damages which may lead to gene mutation and tumor generation. In this report, a microplate-based photoelectrochemical DNA sensor array was developed for the rapid and high throughput screening of DNA damaging chemicals. A 96-well plate with built-in electrodes was fabricated on a plastic substrate by the standard electronics industry processes. The working electrode in each well was deposited with SnO2 nanoparticles, and the resulting film was sintered at low temperatures tolerable for the plastic substrate. The film was characterized by scanning electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. On the plates sintered at 150 degrees C, a significant amount of photocurrent was obtained in a Ru(bpy)(3)(2+) (bpy = 2,2'-bipyridine) solution. To construct a DNA sensor, poly-(diallydimethyl ammonium chloride) and double-stranded DNA were sequentially assembled on the SnO2 electrode by electrostatic interaction, and a DNA intercalator, Ru(bpy)(2)(dppz)(2+) (dppz = dipyrido[3,2-a:2',3'-c]phenazine) was used as the photoelectrochemical signal indicator. After the DNA film was exposed to tetrafluoro-1,4-benzoquinone (TFBQ) or TFBQ/H2O2, the photocurrent dropped by 38% and 73% respectively. The photocurrent reduction can be attributed to less binding of Ru(bpy)(2)(dppz)(2+) to the electrode after DNA damage. Photocurrent measurement of the entire 96-well plate was completed within 22 min automatically. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000301549400043 |
| 公开日期 | 2014-11-27 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/8042]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Yang,Jia, Suping,Guo, Liang-Hong. Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage[J]. Chemistry; Electrochemistry; Instruments & Instrumentation,2012,161(1):334-340. |
| APA | Liu, Yang,Jia, Suping,&Guo, Liang-Hong.(2012).Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage.Chemistry; Electrochemistry; Instruments & Instrumentation,161(1),334-340. |
| MLA | Liu, Yang,et al."Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage".Chemistry; Electrochemistry; Instruments & Instrumentation 161.1(2012):334-340. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。