Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria
文献类型：期刊论文
| 作者 | He, Xiaoqing ; Wei, Yumei ; Cheng, Li ; Zhang, Deyou ; Wang, Zijian |
| 刊名 | ENVIRONMENTAL MONITORING AND ASSESSMENT
 |
| 出版日期 | 2012 |
| 卷号 | 184期号:9页码:5563－5570 |
| 关键词 | Gastroenteritis viruses Fecal indicators Surface water Beijing |
| ISSN号 | 0167-6369 |
| 中文摘要 | To assess the presence of three gastroenteritis viruses responsible for human acute gastroenteritis in surface water, a 1-year study was carried out in the city of Beijing, China. A total of 108 urban surface water samples were collected from nine collection sites which were defined with a global positioning system in rivers or lakes from September 2006 to August 2007. The water samples were subjected to virus concentration using an HA electronegative filter, followed by polymerase chain reaction (PCR) for rotavirus (RV) astrovirus (AV), and norovirus (NV). It showed that the number of viruses detected in water samples from different sites was variable, totaling 63 virus strains, with rotavirus (48.1%) verified as the most prevalent detected, followed by astrovirus (AV, 5.6%), and norovirus (NV, 4.6%). RV was also quantified by real-time PCR and the concentration of RV ranged from 0 to 18.27 genome copies center dot L-1. And the distributions of RV in surface water were abundant in cold weather (from September to February) while less prevailing in warm weather (from March to August). The high detection rate of RV we encountered in this study provided convincing evidence that RV circulated at a certain frequency in the Beijing population. There was no statistically significant correlation between RV levels and both fecal coliform (R (2) = 0.02) and Enterococcus faecalis (R (2) = 0.02) densities. Our study suggests prolonged virus persistence in aquatic environments and emphasizes the enteric virus group as the most reliable for environmental monitoring. |
| WOS记录号 | WOS:000307332300027 |
| 公开日期 | 2014-11-04 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7805]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | He, Xiaoqing,Wei, Yumei,Cheng, Li,et al. Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria[J]. ENVIRONMENTAL MONITORING AND ASSESSMENT,2012,184(9):5563－5570. |
| APA | He, Xiaoqing,Wei, Yumei,Cheng, Li,Zhang, Deyou,&Wang, Zijian.(2012).Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria.ENVIRONMENTAL MONITORING AND ASSESSMENT,184(9),5563－5570. |
| MLA | He, Xiaoqing,et al."Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria".ENVIRONMENTAL MONITORING AND ASSESSMENT 184.9(2012):5563－5570. |
入库方式： OAI收割
来源：生态环境研究中心
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