Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'
文献类型：期刊论文
| 作者 | Wang, Xiaojun; Jin, Liang; Li, Mei; Mao, Minan; Zhao, Haiqing; Xu, Yaozu |
| 刊名 | JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 82期号:5页码:781-785 |
| ISSN号 | 1462-0316 |
| 英文摘要 | An efficient protocol is outlined for the establishment of proliferating cell cluster cultures using a bioreactor, and subsequent regeneration of the aromatic plant, Lavandula angustifolia 'Munstead'. Red calli were obtained by culturing leaflet explants on basal MS medium supplemented with 0.23 mu M 2,4-dichlorophenoxyacetic (2,4-D) and 2.22 mu M 6-benzylaminopurine (BA), and subsequent sub-culturing. Calli were then transferred to basal MS liquid medium containing 0.23 mu M 2,4-D and 2.22 mu M BA to initiate suspension cultures. Cell clusters were obtained by sieving the initial suspensions and sub-culturing them in basal MS liquid medium supplemented with 0.23 mu M 2,4-D plus 2.22 mu M BA and 1.89 mu M abscisic acid, by means of shake culture, or bioreactor culture. After 20 d of culture, cell clusters were transferred to basal MS medium containing 1.14 mu M indole-3-acetic acid and produced a high number of regenerated plants, which were successfully transferred to soil. |
| 收录类别 | SCI |
| WOS记录号 | WOS:000250489300017 |
| 公开日期 | 2014-11-11 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/4118]  |
| 专题 | 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Sch, Beijing 100830, Peoples R China;Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaojun,Jin, Liang,Li, Mei,et al. Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'[J]. JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY,2007,82(5):781-785. |
| APA | Wang, Xiaojun,Jin, Liang,Li, Mei,Mao, Minan,Zhao, Haiqing,&Xu, Yaozu.(2007).Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'.JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY,82(5),781-785. |
| MLA | Wang, Xiaojun,et al."Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'".JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY 82.5(2007):781-785. |
入库方式： OAI收割
来源：新疆理化技术研究所
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