The volatile oil of Nardostachyos Radix et Rhizoma inhibits the oxidative stress-induced cell injury via reactive oxygen species scavenging and Akt activation in H9c2 cardiomyocyte
文献类型：期刊论文
| 作者 | Maiwulanjiang, Maitinuer ; Chen, Jianping; Xin, Guizhong; Gong, Amy G. W.; Miernisha, Abudureyimu; Du, Crystal Y. Q.; Lau, Kei M.; Lee, Pinky S. C.; Chen, Jihang; Dong, Tina T. X.
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2014 |
| 卷号 | 153期号:2页码:491-498 |
| 关键词 | Volatile oil H9c2 cells Oxidative stress Cardioprotection Akt |
| ISSN号 | 0378-8741 |
| 中文摘要 | Ethnopharmacological relevance: Nardostachyos Radix et Rhizoma (NRR; the root and rhizome of Nardostachys jatamansi DC.) is a well-known medicinal herb widely used in Chinese, Uyghur and Ayurvedic medicines for the treatment of cardiovascular disorders. The oxidative stress-induced cardiomyocyte loss is the major pathogenesis of heart disorders. Here, the total volatile oil of NRR was isolated, and its function in preventing the cell death of cardiomyocyte was demonstrated. Materials and methods: The cyto-protective effect of volatile oil of NRR against tBHP-induced H9c2 cardiomyocyte injury was measured by MTT assay. A promoter-report construct (pARE-Luc) containing four repeats of antioxidant response element (ARE) was applied to study the transcriptional activation of ARE. The amounts of phase II antioxidant enzymes were analyzed by quantitative real-time polymer chain reaction (qPCR) upon the volatile oil treatment at 30 mu g/mL for 24 h. The activation of Akt pathway was analyzed by western blot. Results: In cultured H9c2 cardiomyocytes, application of NRR volatile oil exhibited strong potency in preventing tBHP-induced cell death and accumulation of intracellular reactive oxygen species (ROS) in a concentration-dependent manner. In addition, the application of NRR volatile oil in cultures stimulated the gene expressions of self-defense antioxidant enzymes, which was mediated by the transcriptional activation of antioxidant response element (ARE). The induced genes were glutathione S-transferase, NAD(P)H quinone oxidoreductase, glutamate-cysteine ligase catalytic and modulatory subunits. In addition, the volatile oil of NRR activated the phosphotylation of Akt in cultured H9c2 cells. The treatment of LY294002, an Akt inhibitor, significantly inhibited the volatile oil-mediated ARE transcriptional activity, as well as the cell protective effect of NRR oil. Conclusion: These results demonstrated that NRR volatile oil prevented the oxidative stress-induced cell death in H9c2 cells by (i) reducing intracellular ROS production, (ii) inducing antioxidant enzymes and (iii) activating Akt phosphorylation. |
| 收录类别 | SCI |
| 资助信息 | Hong Kong Research Grants Council Theme-based Research Scheme;GRF;Hong Kong Jockey Club Charities Trust Foundation of The Awareness of Nature Lee's Pharmaceutical Co. Ltd. |
| 公开日期 | 2014-11-11 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/3787]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China;Hong Kong Univ Sci & Technol, Ctr Chinese Med, Hong Kong, Hong Kong, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Maiwulanjiang, Maitinuer,Chen, Jianping,Xin, Guizhong,et al. The volatile oil of Nardostachyos Radix et Rhizoma inhibits the oxidative stress-induced cell injury via reactive oxygen species scavenging and Akt activation in H9c2 cardiomyocyte[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014,153(2):491-498. |
| APA | Maiwulanjiang, Maitinuer.,Chen, Jianping.,Xin, Guizhong.,Gong, Amy G. W..,Miernisha, Abudureyimu.,...&Tsim, Karl W. K..(2014).The volatile oil of Nardostachyos Radix et Rhizoma inhibits the oxidative stress-induced cell injury via reactive oxygen species scavenging and Akt activation in H9c2 cardiomyocyte.JOURNAL OF ETHNOPHARMACOLOGY,153(2),491-498. |
| MLA | Maiwulanjiang, Maitinuer,et al."The volatile oil of Nardostachyos Radix et Rhizoma inhibits the oxidative stress-induced cell injury via reactive oxygen species scavenging and Akt activation in H9c2 cardiomyocyte".JOURNAL OF ETHNOPHARMACOLOGY 153.2(2014):491-498. |
入库方式： OAI收割
来源：新疆理化技术研究所
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