Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion
文献类型：期刊论文
| 作者 | Sun GY(孙淦云) ; Zhang Y(章燕); Huo B(霍波); Long M(龙勉); Long M
|
| 刊名 | European Biophysics Journal With Biophysics Letters
 |
| 出版日期 | 2009 |
| 页码 | 701-711 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| ISSN号 | 0175-7571 |
| 关键词 | Association Kinetics Regulating Factors Carrier Diffusion Optical Trap Selectin Ligand Mediated Cell-Adhesion P-Selectin Glycoprotein Ligand-1 Optical Tweezers Shear-Flow Human Neutrophils Force Microscopy Association Rate Contact Area Kinetics |
| 通讯作者 | Long M |
| 中文摘要 | Two-dimensional (2D) kinetics of receptor-ligand interactions governs cell adhesion in many biological processes. While the dissociation kinetics of receptor-ligand bond is extensively investigated, the association kinetics has much less been quantified. Recently receptor-ligand interactions between two surfaces were investigated using a thermal fluctuation assay upon biomembrane force probe technique (Chen et al. in Biophys J 94:694-701, 2008). The regulating factors on association kinetics, however, are not well characterized. Here we developed an alternative thermal fluctuation assay using optical trap technique, which enables to visualize consecutive binding-unbinding transition and to quantify the impact of microbead diffusion on receptor-ligand binding. Three selectin constructs (sLs, sPs, and PLE) and their ligand P-selectin glycoprotein ligand 1 were used to conduct the measurements. It was indicated that bond formation was reduced by enhancing the diffusivity of selectin-coupled carrier, suggesting that carrier diffusion is crucial to determine receptor-ligand binding. It was also found that 2D forward rate predicted upon first-order kinetics was in the order of sPs > sLs > PLE and bond formation was history-dependent. These results further the understandings in regulating association kinetics of surface-bound receptor-ligand interactions. |
| 类目[WOS] | Biophysics |
| 研究领域[WOS] | Biophysics |
| 关键词[WOS] | MEDIATED CELL-ADHESION ; P-SELECTIN ; GLYCOPROTEIN LIGAND-1 ; OPTICAL TWEEZERS ; SHEAR-FLOW ; HUMAN NEUTROPHILS ; FORCE MICROSCOPY ; ASSOCIATION RATE ; CONTACT AREA ; KINETICS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265917300014 |
| 公开日期 | 2009-08-03 ; 2009-10-09 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/26708]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 通讯作者 | Long M |
| 推荐引用方式 GB/T 7714 | Sun GY,Zhang Y,Huo B,et al. Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion[J]. European Biophysics Journal With Biophysics Letters,2009:701-711. |
| APA | 孙淦云,章燕,霍波,龙勉,&Long M.(2009).Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion.European Biophysics Journal With Biophysics Letters,701-711. |
| MLA | 孙淦云,et al."Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion".European Biophysics Journal With Biophysics Letters (2009):701-711. |
入库方式： OAI收割
来源：力学研究所
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