Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency
文献类型：期刊论文
| 作者 | Yang F(杨帆); Gao YX(高宇欣) ; Zhang Y(章燕); Chen J(陈娟); Long M(龙勉); Long M
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| 刊名 | Science in China Series C-Life Sciences
 |
| 出版日期 | 2009 |
| 卷号 | 52期号:2页码:173-181 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| ISSN号 | 1006-9305 |
| 关键词 | Cell Surface Marker Microfluidic Capture Efficiency Ligand Binding-Kinetics Antibody Microarray Surface-Antigens Leukemias Identification Diagnostics Membrane Platform Design Marker |
| 通讯作者 | Long M |
| 中文摘要 | Micro-fabrication technology has substantial potential for identifying molecular markers expressed on the surfaces of tissue cells and viruses. It has been found in several conceptual prototypes that cells with such markers are able to be captured by their antibodies immobilized on microchannel substrates and unbound cells are flushed out by a driven flow. The feasibility and reliability of such a microfluidic-based assay, however, remains to be further tested. In the current work, we developed a microfluidic-based system consisting of a microfluidic chip, an image grabbing unit, data acquisition and analysis software, as well as a supporting base. Specific binding of CD59-expressed or BSA-coupled human red blood cells (RBCs) to anti-CD59 or anti-BSA antibody-immobilized chip surfaces was quantified by capture efficiency and by the fraction of bound cells. Impacts of respective flow rate, cell concentration, antibody concentration and site density were tested systematically. The measured data indicated that the assay was robust. The robustness was further confirmed by capture efficiencies measured from an independent ELISA-based cell binding assay. These results demonstrated that the system developed provided a new platform to effectively quantify cellular surface markers effectively, which promoted the potential applications in both biological studies and clinical diagnoses. |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | LIGAND BINDING-KINETICS ; ANTIBODY MICROARRAY ; SURFACE-ANTIGENS ; LEUKEMIAS ; IDENTIFICATION ; DIAGNOSTICS ; MEMBRANE ; PLATFORM ; DESIGN ; MARKER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264106000017 |
| 公开日期 | 2009-08-03 ; 2009-10-09 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/26718]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 通讯作者 | Long M |
| 推荐引用方式 GB/T 7714 | Yang F,Gao YX,Zhang Y,et al. Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency[J]. Science in China Series C-Life Sciences,2009,52(2):173-181. |
| APA | 杨帆,高宇欣,章燕,陈娟,龙勉,&Long M.(2009).Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency.Science in China Series C-Life Sciences,52(2),173-181. |
| MLA | 杨帆,et al."Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency".Science in China Series C-Life Sciences 52.2(2009):173-181. |
入库方式： OAI收割
来源：力学研究所
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