Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium
文献类型：期刊论文
| 作者 | Wang, Xiaohui1,2,3,5; Zhao, Yong1; Tan, Haidong1; Chi, Naiyu2; Zhang, Qingfang2; Du, Yuguang4; Yin, Heng1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 70期号:SEP.页码:455-462 |
| 关键词 | Chitinase Purification Characterisation |
| ISSN号 | 0141-8130 |
| 其他题名 | Int. J. Biol. Macromol. |
| 中文摘要 | In this study, we isolated a new psychrophilic bacterium, Pseudoalteromonas sp. DL-6 from marine sediments, which grew well on chitin-containing plates at 4 degrees C. One endo-type chitinase gene, chiA, was cloned from the genomic DNA of this bacterium and heterologously expressed in Escherichia coli BL21 (DE3). ChiA showed very high catalytic activity, even at 4 degrees C, and exhibited maximal activity on a chitinous substrate at pH 8.0 and 20 degrees C. Kinetic studies indicated that ChiA has a greater catalytic efficiency on 4-methylumbelliferyl-beta-D-N,N',N"-triacetylchitotriose[4-MU(GlcNAc)(3)] than on 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside[4-MU(GlcNAc)(2)]. Electrospray ionisation mass spectrometry (ESI-MS) analysis showed that the hydrolysis products of powdered chitin after ChiA digestion consisted of a series of chitin oligomers with different degrees of polymerisation. The ChiA mode of action was also examined using (GlcNAc)(2-6) as a substrate, and the results suggested that ChiA is a non-processive endo-type chitinase. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | In this study, we isolated a new psychrophilic bacterium, Pseudoalteromonas sp. DL-6 from marine sediments, which grew well on chitin-containing plates at 4 degrees C. One endo-type chitinase gene, chiA, was cloned from the genomic DNA of this bacterium and heterologously expressed in Escherichia coli BL21 (DE3). ChiA showed very high catalytic activity, even at 4 degrees C, and exhibited maximal activity on a chitinous substrate at pH 8.0 and 20 degrees C. Kinetic studies indicated that ChiA has a greater catalytic efficiency on 4-methylumbelliferyl-beta-D-N,N',N"-triacetylchitotriose[4-MU(GlcNAc)(3)] than on 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside[4-MU(GlcNAc)(2)]. Electrospray ionisation mass spectrometry (ESI-MS) analysis showed that the hydrolysis products of powdered chitin after ChiA digestion consisted of a series of chitin oligomers with different degrees of polymerisation. The ChiA mode of action was also examined using (GlcNAc)(2-6) as a substrate, and the results suggested that ChiA is a non-processive endo-type chitinase. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SP STRAIN O-7 ; SERRATIA-MARCESCENS ; RECALCITRANT POLYSACCHARIDES ; FAMILY-18 CHITINASE ; MOLECULAR ANALYSIS ; MORITELLA-MARINA ; BINDING DOMAIN ; GENE ; PROCESSIVITY ; DEGRADATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000342478800065 |
| 公开日期 | 2014-11-02 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11658]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China 3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaohui,Zhao, Yong,Tan, Haidong,et al. Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2014,70(SEP.):455-462. |
| APA | Wang, Xiaohui.,Zhao, Yong.,Tan, Haidong.,Chi, Naiyu.,Zhang, Qingfang.,...&Yin, Heng.(2014).Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,70(SEP.),455-462. |
| MLA | Wang, Xiaohui,et al."Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 70.SEP.(2014):455-462. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。