A Trabecular Bone Explant Model of Osteocyte–Osteoblast Co-Culture for Bone Mechanobiology
文献类型：期刊论文
| 作者 | Chan ME; Lu XL; Huo B(霍波) ; Baik AD; Chiang V; Guldberg RE; Lu HH; Guo XE
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| 刊名 | Cellular and Molecular Bioengineering
 |
| 出版日期 | 2009 |
| 卷号 | 2期号:3页码:405-415 |
| 通讯作者邮箱 | ed.guo@columbia.edu |
| ISSN号 | 1865-5025 |
| 通讯作者 | Guo XE |
| 合作状况 | 国际 |
| 中文摘要 | The osteocyte network is recognized as the major mechanical sensor in the bone remodeling process, and osteocyte-osteoblast communication acts as an important mediator in the coordination of bone formation and turnover. In this study, we developed a novel 3D trabecular bone explant co-culture model that allows live osteocytes situated in their native extracellular matrix environment to be interconnected with seeded osteoblasts on the bone surface. Using a low-level medium perfusion system, the viability of in situ osteocytes in bone explants was maintained for up to 4 weeks, and functional gap junction intercellular communication (GJIC) was successfully established between osteocytes and seeded primary osteoblasts. Using this novel co-culture model, the effects of dynamic deformational loading, GJIC, and prostaglandin E-2 (PGE(2)) release on functional bone adaptation were further investigated. The results showed that dynamical deformational loading can significantly increase the PGE(2) release by bone cells, bone formation, and the apparent elastic modulus of bone explants. However, the inhibition of gap junctions or the PGE(2) pathway dramatically attenuated the effects of mechanical loading. This 3D trabecular bone explant co-culture model has great potential to fill in the critical gap in knowledge regarding the role of osteocytes as a mechano-sensor and how osteocytes transmit signals to regulate osteoblasts function and skeletal integrity as reflected in its mechanical properties. |
| 学科主题 | 生物力学 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270168900014 |
| 公开日期 | 2009-12-30 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/33019]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Chan ME,Lu XL,Huo B,et al. A Trabecular Bone Explant Model of Osteocyte–Osteoblast Co-Culture for Bone Mechanobiology[J]. Cellular and Molecular Bioengineering,2009,2(3):405-415. |
| APA | Chan ME.,Lu XL.,霍波.,Baik AD.,Chiang V.,...&Guo XE.(2009).A Trabecular Bone Explant Model of Osteocyte–Osteoblast Co-Culture for Bone Mechanobiology.Cellular and Molecular Bioengineering,2(3),405-415. |
| MLA | Chan ME,et al."A Trabecular Bone Explant Model of Osteocyte–Osteoblast Co-Culture for Bone Mechanobiology".Cellular and Molecular Bioengineering 2.3(2009):405-415. |
入库方式： OAI收割
来源：力学研究所
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