Fluid flow induced calcium response in bone cell network
文献类型：期刊论文
| 作者 | Huo B(霍波); Lu XL; Hung CT; Costa KD; Xu Q; Whitesides GM; Guo XE; Huo B(霍波)
|
| 刊名 | Cellular and Molecular Bioengineering
 |
| 出版日期 | 2008 |
| 卷号 | 1期号:1页码:58-66 |
| 通讯作者邮箱 | ed.guo@columbia.edu |
| ISSN号 | 1865-5025 |
| 通讯作者 | Guo XE |
| 合作状况 | 国际 |
| 中文摘要 | In our previous work, bone cell networks with controlled spacing and functional intercellular gap junctions had been successfully established by using microcontact printing and self assembled monolayers technologies [Guo, X. E., E. Takai, X. Jiang, Q. Xu, G. M. Whitesides, J. T. Yardley, C. T. Hung, E. M. Chow, T. Hantschel, and K. D. Costa. Mol. Cell. Biomech. 3:95-107, 2006]. The present study investigated the calcium response and the underlying signaling pathways in patterned bone cell networks exposed to a steady fluid flow. The glass slides with cell networks were separated into eight groups for treatment with specific pharmacological agents that inhibit pathways significant in bone cell calcium signaling. The calcium transients of the network were recorded and quantitatively evaluated with a set of network parameters. The results showed that 18 alpha-GA (gap junction blocker), suramin (ATP inhibitor), and thapsigargin (depleting intracellular calcium stores) significantly reduced the occurrence of multiple calcium peaks, which were visually obvious in the untreated group. The number of responsive peaks also decreased slightly yet significantly when either the COX-2/PGE(2) or the NOS/nitric oxide pathway was disrupted. Different from all other groups, cells treated with 18 alpha-GA maintained a high concentration of intracellular calcium following the first peak. In the absence of calcium in the culture medium, the intracellular calcium concentration decreased slowly with fluid flow without any calcium transients observed. These findings have identified important factors in the flow mediated calcium signaling of bone cells within a patterned network. |
| 学科主题 | 生物力学 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269821600009 |
| 公开日期 | 2009-12-30 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/33020]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Huo B,Lu XL,Hung CT,et al. Fluid flow induced calcium response in bone cell network[J]. Cellular and Molecular Bioengineering,2008,1(1):58-66. |
| APA | 霍波.,Lu XL.,Hung CT.,Costa KD.,Xu Q.,...&Huo B.(2008).Fluid flow induced calcium response in bone cell network.Cellular and Molecular Bioengineering,1(1),58-66. |
| MLA | 霍波,et al."Fluid flow induced calcium response in bone cell network".Cellular and Molecular Bioengineering 1.1(2008):58-66. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。