中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Fluid flow induced calcium response in bone cell network

文献类型:期刊论文

作者Huo B(霍波); Lu XL; Hung CT; Costa KD; Xu Q; Whitesides GM; Guo XE; Huo B(霍波)
刊名Cellular and Molecular Bioengineering
出版日期2008
卷号1期号:1页码:58-66
通讯作者邮箱ed.guo@columbia.edu
ISSN号1865-5025
通讯作者Guo XE
合作状况国际
中文摘要In our previous work, bone cell networks with controlled spacing and functional intercellular gap junctions had been successfully established by using microcontact printing and self assembled monolayers technologies [Guo, X. E., E. Takai, X. Jiang, Q. Xu, G. M. Whitesides, J. T. Yardley, C. T. Hung, E. M. Chow, T. Hantschel, and K. D. Costa. Mol. Cell. Biomech. 3:95-107, 2006]. The present study investigated the calcium response and the underlying signaling pathways in patterned bone cell networks exposed to a steady fluid flow. The glass slides with cell networks were separated into eight groups for treatment with specific pharmacological agents that inhibit pathways significant in bone cell calcium signaling. The calcium transients of the network were recorded and quantitatively evaluated with a set of network parameters. The results showed that 18 alpha-GA (gap junction blocker), suramin (ATP inhibitor), and thapsigargin (depleting intracellular calcium stores) significantly reduced the occurrence of multiple calcium peaks, which were visually obvious in the untreated group. The number of responsive peaks also decreased slightly yet significantly when either the COX-2/PGE(2) or the NOS/nitric oxide pathway was disrupted. Different from all other groups, cells treated with 18 alpha-GA maintained a high concentration of intracellular calcium following the first peak. In the absence of calcium in the culture medium, the intracellular calcium concentration decreased slowly with fluid flow without any calcium transients observed. These findings have identified important factors in the flow mediated calcium signaling of bone cells within a patterned network.
学科主题生物力学
收录类别SCI
语种英语
WOS记录号WOS:000269821600009
公开日期2009-12-30
源URL[http://dspace.imech.ac.cn/handle/311007/33020]  
专题力学研究所_力学所知识产出(1956-2008)
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GB/T 7714
Huo B,Lu XL,Hung CT,et al. Fluid flow induced calcium response in bone cell network[J]. Cellular and Molecular Bioengineering,2008,1(1):58-66.
APA 霍波.,Lu XL.,Hung CT.,Costa KD.,Xu Q.,...&Huo B.(2008).Fluid flow induced calcium response in bone cell network.Cellular and Molecular Bioengineering,1(1),58-66.
MLA 霍波,et al."Fluid flow induced calcium response in bone cell network".Cellular and Molecular Bioengineering 1.1(2008):58-66.

入库方式: OAI收割

来源:力学研究所

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