中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 力学研究所
  3. 中国科学院力学研究所
  4. 国家微重力实验室
Parametric Analysis for Monitoring 2D Kinetics of Receptor-Ligand binding

文献类型：期刊论文

作者Sun GY(孙淦云); Zhang Y(章燕); Huo B(霍波); Long M(龙勉)
刊名Cellular and Molecular Bioengineering
出版日期2009
卷号2期号:4页码:495-503
通讯作者邮箱mlong@imech.ac.cn
ISSN号1865-5025
通讯作者龙勉
合作状况其它
中文摘要Thermal fluctuation approach is widely used to monitor association kinetics of surface-bound receptor-ligand interactions. Various protocols such as sliding standard deviation (SD) analysis (SSA) and Page's test analysis (PTA) have been used to estimate two-dimensional (2D) kinetic rates from the time course of displacement of molecular carrier. In the current work, we compared the estimations from both SSA and modified PTA using measured data from an optical trap assay and simulated data from a random number generator. Our results indicated that both SSA and PTA were reliable in estimating 2D kinetic rates. Parametric analysis also demonstrated that such the estimations were sensitive to parameters such as sampling rate, sliding window size, and threshold. These results furthered the understandings in quantifying the biophysics of receptor-ligand interactions.
学科主题生物力学
类目[WOS]Cell & Tissue Engineering ; Biophysics ; Cell Biology
研究领域[WOS]Cell Biology ; Biophysics
关键词[WOS]L-SELECTIN ; ADHESION MOLECULES ; OPTICAL TWEEZERS ; P-SELECTIN ; FORCE ; BONDS ; SHEAR ; FLOW ; FLUCTUATIONS ; DISSOCIATION
收录类别SCI
语种英语
WOS记录号WOS:000272671600005
公开日期2009-12-30
源URL[http://dspace.imech.ac.cn/handle/311007/33023]  
专题力学研究所_国家微重力实验室
推荐引用方式
GB/T 7714		 Sun GY,Zhang Y,Huo B,et al. Parametric Analysis for Monitoring 2D Kinetics of Receptor-Ligand binding[J]. Cellular and Molecular Bioengineering,2009,2(4):495-503.
APA 孙淦云,章燕,霍波,&龙勉.(2009).Parametric Analysis for Monitoring 2D Kinetics of Receptor-Ligand binding.Cellular and Molecular Bioengineering,2(4),495-503.
MLA 孙淦云,et al."Parametric Analysis for Monitoring 2D Kinetics of Receptor-Ligand binding".Cellular and Molecular Bioengineering 2.4(2009):495-503.

入库方式： OAI收割

来源：力学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。