Membrane Deformability and Membrane Tension of Single Isolated Mitochondria
文献类型：期刊论文
| 作者 | Wang SQ(王世骐); Jiang CS; Zhang Y(章燕); Chen J(陈娟); Wang B; Long M(龙勉); Chen J(陈娟) ; Zhang Y(章燕); Long M(龙勉); Zhang Y(章燕)
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| 刊名 | Cellular And Molecular Bioengineering
 |
| 出版日期 | 2008 |
| 卷号 | 1期号:1页码:67-74 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| ISSN号 | 1865-5025 |
| 关键词 | Mechanical Properties Physical-Chemical Environment Respiratory State Membrane Permeability Transition Cytochrome-C Oxidative-Phosphorylation Permeability Transition Mechanisms Release |
| 通讯作者 | Long M |
| 合作状况 | 国内 |
| 中文摘要 | Mitochondria dynamics is crucial to many biological processes such as mitochondria fusion and fission, which is highly correlated to the mechanics of single mitochondria. However, the mechanobiological coupling of mitochondria has been poorly understood. Here membrane deformability and membrane tension of individual mitochondria isolated from MtDsRed labeled human embryonic T-Rex-293 kidney cells were measured using a micropipette aspiration assay. The results demonstrated that membrane deformation of isolated mitochondria exhibited an elastic transition phase followed by an equilibrium phase, and mitochondrial membrane tension was proportional to the area compressibility. It was also indicated that mitochondrial membrane deformability was significantly affected by physical chemical factors such as osmotic pressure or pH value, and was further correlated to mitochondrial functionality in different respiratory states and Ca2+ regulation. These findings provide a new insight into understanding the mechanical regulation of mitochondrial physiology. |
| 资助信息 | National Natural Science Foundation of China [10332060, 30730032]; National Key Basic Research Foundation of China [2006CB910303]; Chinese Academy of Sciences [2005-1-16] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269821600010 |
| 公开日期 | 2009-08-03 ; 2010-06-03 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/33565]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 通讯作者 | Long M |
| 推荐引用方式 GB/T 7714 | Wang SQ,Jiang CS,Zhang Y,et al. Membrane Deformability and Membrane Tension of Single Isolated Mitochondria[J]. Cellular And Molecular Bioengineering,2008,1(1):67-74. |
| APA | 王世骐.,Jiang CS.,章燕.,陈娟.,Wang B.,...&Zhang Y.(2008).Membrane Deformability and Membrane Tension of Single Isolated Mitochondria.Cellular And Molecular Bioengineering,1(1),67-74. |
| MLA | 王世骐,et al."Membrane Deformability and Membrane Tension of Single Isolated Mitochondria".Cellular And Molecular Bioengineering 1.1(2008):67-74. |
入库方式： OAI收割
来源：力学研究所
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