Development of SNP markers in Crassostrea hongkongensis based on the next-generation sequencing and high resolution melting analysis
文献类型：期刊论文
| 作者 | Huang, JM ; Zhang, Y ; Li, J ; Yu, ZN |
| 刊名 | CONSERVATION GENETICS RESOURCES
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:3页码:559-562 |
| 关键词 | C. hongkongensis SNP markers Transcriptome sequencing High resolution melting |
| ISSN号 | 1877-7252 |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 中文摘要 | Novel single nucleotide polymorphisms (SNPs) were screened using next generation sequencing in Hong Kong oyster, Crassostrea hongkongensis. A total of 91,980 putative SNPs were identified through de novo assembly of the 454 transcriptome database, and 83 of these were selected to validate polymorphism. Using high resolution melting analysis, 17 SNP markers were developed successfully with 48 individuals from two wild populations from Shantou and Zhuhai, China. The observed heterozygosities ranged from 0.125 to 0.667 and 0 to 0.708 in the two populations, respectively, while the expected ones ranged from 0.311 to 0.511 and 0.120 to 0.511 in the two samples, respectively. Fifteen SNPs have been confirmed to fit Hardy-Weinberg Equilibrium after Bonferroni correction (p < 0.003) in both populations. To our knowledge, this is the first set of SNP markers developed in C. hongkongensis, which would provide valuable information in facilitating genetics and breeding studies in the future. |
| 学科主题 | Biodiversity & Conservation ; Genetics & Heredity |
| 收录类别 | SCI |
| 资助信息 | National High-Tech Research and Development Program of China [2012AA10A405-3] |
| 原文出处 | SPRINGER |
| WOS记录号 | WOS:000340404900016 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10511]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Huang, JM,Zhang, Y,Li, J,et al. Development of SNP markers in Crassostrea hongkongensis based on the next-generation sequencing and high resolution melting analysis[J]. CONSERVATION GENETICS RESOURCES,2014,6(3):559-562. |
| APA | Huang, JM,Zhang, Y,Li, J,&Yu, ZN.(2014).Development of SNP markers in Crassostrea hongkongensis based on the next-generation sequencing and high resolution melting analysis.CONSERVATION GENETICS RESOURCES,6(3),559-562. |
| MLA | Huang, JM,et al."Development of SNP markers in Crassostrea hongkongensis based on the next-generation sequencing and high resolution melting analysis".CONSERVATION GENETICS RESOURCES 6.3(2014):559-562. |
入库方式： OAI收割
来源：南海海洋研究所
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