A novel ortholog of serum response factor (SRF) with immune defense function identified in Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Xiang, ZM ; Qu, FF ; Qi, L ; Zhang, Y ; Xiao, S ; Yu, ZN |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 36期号:1页码:75-82 |
| ISSN号 | 1050-4648 |
| 关键词 | Crassostrea hongkongensis ChSRF MAPK signal pathway |
| 通讯作者 | zhimingxiang@scsio.ac.cn ; carlzyu@scsio.ac.cn |
| 中文摘要 | Serum response factor (SRF) function is essential for transcriptional regulation of numerous growth-factor-inducible genes and triggers proliferation, differentiation and apoptosis of the cells. In this report, the first mollusk serum response factor like homolog gene (designated ChSRF) was identified and characterized from the Hong Kong oyster, Crassostrea hongkongensis. The full-length cDNA of ChSRF was 1716 bp in length and encodes a putative protein of 434 amino acids respectively, and shares the MADS domain at the N-terminal. ChSRF is ubiquitously expressed in various tissues, with the highest expression level observed in muscle. Temporal expression of ChSRF following microbe infection shows that the expression of ChSRF in hemocytes increases from 3 to 24 h post-challenge. As a target gene of SRF, beta-actin demonstrates a similar gene expression mode in constitutive tissue and pathogen infection. Furthermore, some protein profiles of ChSRF was revealed, fluorescence microscopy results show that ChSRF located in the nuclei of HeLa cells and over-expression of ChSRF activated the transcriptional activities of MAPK signal pathway in HEK293T cells. These results indicate that ChSRF maybe play an important role in signal transduction in the immunity and development response of oysters. Furthermore, we found that ChSRF could regulate the expression of beta-actin gene, which indicate that ChSRF is a muscle differentiation regulator in the oyster and it will help us to improve aquaculture production. (C) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 资助信息 | National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] |
| 收录类别 | SCI |
| 原文出处 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000331671600010 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10589]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Xiang, ZM,Qu, FF,Qi, L,et al. A novel ortholog of serum response factor (SRF) with immune defense function identified in Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2014,36(1):75-82. |
| APA | Xiang, ZM,Qu, FF,Qi, L,Zhang, Y,Xiao, S,&Yu, ZN.(2014).A novel ortholog of serum response factor (SRF) with immune defense function identified in Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,36(1),75-82. |
| MLA | Xiang, ZM,et al."A novel ortholog of serum response factor (SRF) with immune defense function identified in Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 36.1(2014):75-82. |
入库方式： OAI收割
来源：南海海洋研究所
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