Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China
文献类型：期刊论文
| 作者 | Wu, P ; Wang, YS ; Sun, FL ; Wu, ML ; Peng, YL |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 98期号:2页码:875-884 |
| 关键词 | Polycyclic aromatic hydrocarbons Ring-hydroxylating dioxygenase The Pearl River estuary Bacterial community |
| ISSN号 | 0175-7598 |
| 通讯作者 | yswang@scsio.ac.cn |
| 中文摘要 | Bacterial community compositions were characterized using denaturing gradient gel electrophoresis analysis of bacterial 16S rRNA gene in the sediments of the Pearl River estuary. Sequencing analyses of the excised bands indicated that Gram-negative bacteria, especially Gammaproteobacteria, were dominant in the Pearl River estuary. The diversity of polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) gene in this estuary was then assessed by clone library analysis. The phylogenetic analyses showed that all PAH-RHD gene sequences of Gram-negative bacteria (PAH-RHD[GN]) were closely related to the nagAc gene described for Ralstonia sp. U2 or nahAc gene for Pseudomonas sp. 9816-4, while the PAH-RHD gene sequences of Gram-positive bacteria (PAH-RHD[GP]) at sampling site A1 showed high sequence similarity to the nidA gene from Mycobacterium species. Meanwhile, molecular diversity of the two functional genes was higher at the upstream of this region, while lower at the downstream. Redundancy analysis indicated that environmental factors, such as NH4-N, aPAHs, pH, SiO3-Si, and water depth, affected the distribution of the PAH-RHD[GN] gene in the Pearl River estuary. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 资助信息 | Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] |
| 原文出处 | SPRINGER |
| WOS记录号 | WOS:000329627600035 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10594]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Wu, P,Wang, YS,Sun, FL,et al. Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(2):875-884. |
| APA | Wu, P,Wang, YS,Sun, FL,Wu, ML,&Peng, YL.(2014).Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(2),875-884. |
| MLA | Wu, P,et al."Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.2(2014):875-884. |
入库方式： OAI收割
来源：南海海洋研究所
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