Insights into Deep-Sea Sediment Fungal Communities from the East Indian Ocean Using Targeted Environmental Sequencing Combined with Traditional Cultivation
文献类型：期刊论文
| 作者 | Zhang, XY ; Tang, GL ; Xu, XY ; Nong, XH ; Qi, SH |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:10页码:-e109118 |
| ISSN号 | 1932-6203 |
| 通讯作者 | shuhuaqi@scsio.ac.cn |
| 中文摘要 | The fungal diversity in deep-sea environments has recently gained an increasing amount attention. Our knowledge and understanding of the true fungal diversity and the role it plays in deep-sea environments, however, is still limited. We investigated the fungal community structure in five sediments from a depth of,4000 m in the East India Ocean using a combination of targeted environmental sequencing and traditional cultivation. This approach resulted in the recovery of a total of 45 fungal operational taxonomic units (OTUs) and 20 culturable fungal phylotypes. This finding indicates that there is a great amount of fungal diversity in the deep-sea sediments collected in the East Indian Ocean. Three fungal OTUs and one culturable phylotype demonstrated high divergence (89%-97%) from the existing sequences in the GenBank. Moreover, 44.4% fungal OTUs and 30% culturable fungal phylotypes are new reports for deep-sea sediments. These results suggest that the deep-sea sediments from the East India Ocean can serve as habitats for new fungal communities compared with other deep-sea environments. In addition, different fungal community could be detected when using targeted environmental sequencing compared with traditional cultivation in this study, which suggests that a combination of targeted environmental sequencing and traditional cultivation will generate a more diverse fungal community in deep-sea environments than using either targeted environmental sequencing or traditional cultivation alone. This study is the first to report new insights into the fungal communities in deep-sea sediments from the East Indian Ocean, which increases our knowledge and understanding of the fungal diversity in deep-sea environments. |
| 学科主题 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03] |
| 原文出处 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000343729600098 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10372]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, XY,Tang, GL,Xu, XY,et al. Insights into Deep-Sea Sediment Fungal Communities from the East Indian Ocean Using Targeted Environmental Sequencing Combined with Traditional Cultivation[J]. PLOS ONE,2014,9(10):-e109118. |
| APA | Zhang, XY,Tang, GL,Xu, XY,Nong, XH,&Qi, SH.(2014).Insights into Deep-Sea Sediment Fungal Communities from the East Indian Ocean Using Targeted Environmental Sequencing Combined with Traditional Cultivation.PLOS ONE,9(10),-e109118. |
| MLA | Zhang, XY,et al."Insights into Deep-Sea Sediment Fungal Communities from the East Indian Ocean Using Targeted Environmental Sequencing Combined with Traditional Cultivation".PLOS ONE 9.10(2014):-e109118. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。