Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas
文献类型：期刊论文
| 作者 | Li, J ; Zhang, Y ; Zhang, YH ; Xiang, ZM ; Tong, Y ; Qu, FF ; Yu, ZN |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 40期号:2页码:455-465 |
| 关键词 | Interleukin-17 Genomic organization PAMPs challenge Pacific oyster |
| ISSN号 | 1050-4648 |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 中文摘要 | Interleukin-17 (IL-17) is a proinflammatory cytokine that plays an important role in clearing extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. In the present study, five novel IL-17 homologs were identified by searching and analyzing the Pacific oyster genome. All six CgIL-17 members (including a previously reported homolog) contained four conserved cysteines that were used in the formation of disulfide bonds. Phylogenetic analysis showed that all invertebrate IL-17s were clustered into one group, implying that invertebrate IL-17s evolved from one common ancestral gene and subsequently diversified. All CgIL-17s shared the same genomic structure, containing two exons and one intron, except for the CgIL-17-3 and CgIL-17-5 genes, which each had only one exon. The expression pattern of the CgIL-17 genes was analyzed by gRT-PCR in a variety of tissues and at different developmental stages, and these genes were highly expressed in the gill and digestive gland tissues. Moreover, the expression of the CgIL-17 family genes was significantly up-regulated in hemocytes challenged with Pathogen-Associated Molecular Patterns (PAMPs). CgIL-17-3 had a strong response to lipopolysaccharide (LPS) and heat-killed Vibrio alginolyticus (HKVA) challenge, while CgIL-17-5 and CgIL-17-6 can be activated by peptidoglycan (PGN), but not by heat-killed Listeria monogtogenes (HKLM). The distinct, up-regulated transcript levels of the CgIL-17s in response to PAMPs challenge further indicate that CgIL-17s are likely to be significant components of immune responses by playing diversified roles in host defense in the Pacific oyster. These findings suggest that CgIL-17s are involved in innate immune responses and further supports their conserved function in mollusks immunity. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215] |
| 原文出处 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000343381100014 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10374]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, J,Zhang, Y,Zhang, YH,et al. Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):455-465. |
| APA | Li, J.,Zhang, Y.,Zhang, YH.,Xiang, ZM.,Tong, Y.,...&Yu, ZN.(2014).Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,40(2),455-465. |
| MLA | Li, J,et al."Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):455-465. |
入库方式： OAI收割
来源：南海海洋研究所
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