Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity
文献类型：期刊论文
| 作者 | Zhang, Y ; Li, J ; Yu, F ; Tong, Y ; Zhang, YH ; Xiang, ZM ; Yu, ZN |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 40期号:1页码:239-244 |
| 关键词 | Crassostrea gigas SIMPL Ancient origin Rel/NF-kappa B transcription activity |
| ISSN号 | 1050-4648 |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 中文摘要 | SIMPL (Signaling Molecule that associates with the mouse Pelle-Like Kinase) has been recently identified as a co-regulator of NF-kappa B dependent transcription. Here, we report the discovery and functional analysis of the SIMPL in a mollusk, Crassostrea gigas, which terms as CgSIMPL. CgSIMPL is comprised of 252 amino acids and shares significant homology with vertebrate homologs. Over-expression of CgSIMPL does not activate the NF-kappa B reporter in the HEK293 cell line, but can enhance Rel-dependent NF-kappa B transactivation. The dominant-negative effect of CgSIMPL was observed after the deletion of NLS, strongly suggesting that NLS is required for the enhancement of Rel-dependent NF-kappa B transactivation. Furthermore, CgSIMPL mRNA is constitutively expressed in various tissues and is inducible at late stages of infection, supporting its regulatory function in innate immunity. Altogether, our studies reveal that SIMPL is reserved and co-evolved with NF-kappa B in chordate and mollusk, supporting its ancient origin and involvement in regulation of NF-kappa B signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] |
| 原文出处 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000341468500028 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10389]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Li, J,Yu, F,et al. Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(1):239-244. |
| APA | Zhang, Y.,Li, J.,Yu, F.,Tong, Y.,Zhang, YH.,...&Yu, ZN.(2014).Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity.FISH & SHELLFISH IMMUNOLOGY,40(1),239-244. |
| MLA | Zhang, Y,et al."Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity".FISH & SHELLFISH IMMUNOLOGY 40.1(2014):239-244. |
入库方式： OAI收割
来源：南海海洋研究所
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